PCR Mutation Detection Protocols

. Ed(s): Theophilus, Bimal D.M.; Rapley, Ralph

ISBN 10: 1607619466 / ISBN 13: 9781607619468

Published by Humana Press Inc., 2010

The Second Edition updates the subject with key protocols involving the PCR and its many incarnations such as SSCP, CSGE, and dHPLC. The book also addresses such areas as Southern blotting, accurate diagnostics with high throughput, and microarray systems. Editor(s): Theophilus, Bimal D.M.; Rapley, Ralph. Series: Methods in Molecular Biology. Num Pages: 298 pages, 18 black & white tables, biography. BIC Classification: PB. Category: (P) Professional & Vocational. Dimension: 264 x 186 x 28. Weight in Grams: 746. . 2010. 2nd ed. 2011. Hardback. . . . . Seller Inventory # V9781607619468

Synopsis:

Since the publication of the popular first edition, the explosion of DNA sequence information, the access to bioinformatics and mutation databases coupled with the ability to readily detect and confirm mutations has cemented the role of molecular diagnostics in medicine and, in particular, mutation detection by the polymerase chain reaction (PCR). In PCR Mutation Detection Protocols, Second Edition, expert researchers bring the subject up-to-date with key protocols involving the PCR and its many various incarnations such as SSCP, CSGE, and dHPLC. The volume also addresses key areas such as Southern blotting, accurate diagnostics with high throughput, as well as microarray systems. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes which provide the often hard to find information that may mean the difference between the success and failure of the method. Authoritative and cutting-edge, PCR Mutation Detection Protocols, Second Edition aims to stimulate postgraduate scientists, researchers, and clinicians already engaged in the area and to provide an important first step for those new to this practice wanting to adopt the powerful and essential technique in their own laboratories.

From the Back Cover: Since the publication of the popular first edition, the explosion of DNA sequence information, the access to bioinformatics and mutation databases coupled with the ability to readily detect and confirm mutations has cemented the role of molecular diagnostics in medicine and, in particular, mutation detection by the polymerase chain reaction (PCR). In PCR Mutation Detection Protocols, Second Edition, expert researchers bring the subject up-to-date with key protocols involving the PCR and its many various incarnations such as SSCP, CSGE, and dHPLC. The volume also addresses key areas such as Southern blotting, accurate diagnostics with high throughput, as well as microarray systems. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes which provide the often hard to find information that may mean the difference between the success and failure of the method. Authoritative and cutting-edge, PCR Mutation Detection Protocols, Second Edition aims to stimulate postgraduate scientists, researchers, and clinicians already engaged in the area and to provide an important first step for those new to this practice wanting to adopt the powerful and essential technique in their own laboratories.

Bibliographic Details

Title: PCR Mutation Detection Protocols
Publisher: Humana Press Inc.
Publication Date: 2010
Binding: Soft cover
Condition: New
Edition: 2nd Edition