PCR/RT- PCR in situ : Light and Electron Microscopy

Gerard (Universite Claude Bernard Morel

ISBN 10: 084930041X / ISBN 13: 9780849300417

Published by Taylor & Francis Inc Sep 2002, 2002

About this Item

Neuware - This book covers methods of in situ polymerase chain reaction (PCR) and reverse transcription PCR (RT-PCR), two new approaches in visualizing very low amounts of DNA and mRNA in tissues and cell cultures at the light and electron microscope levels. Written by experts in this field, the text provides theoretical consideration, as well as practical approaches to in situ PCR. Organized in a user-friendly two-column format, it provides step-by-step protocols, preparation of reagents and solutions, and appropriate controls to allow the researcher to perform and optimize these sensitive and powerful techniques.

Seller Inventory # 9780849300417

Bibliographic Details

• Title: PCR/RT- PCR in situ : Light and Electron Microscopy
• Publisher: Taylor & Francis Inc Sep 2002
• Publication Date: 2002
• Language: English
• ISBN 10: 084930041X
• ISBN 13: 9780849300417
• Binding: Buch
• Condition: Neu
• Dimensions: 254W x 178H x 0D millimeters 254 millimeters width by 178 millimeters height by 0 millimeters depth
• Weight: 1014 grams

About this title

Synopsis

Although the polymerase chain reaction has revolutionized genetic analysis by amplifying rare nucleic acid sequences, the in situ application is the only method that allows the localization of amplified signal within tissue structure. The applications of in situ polymerase chain reaction have greatly enhanced the field of investigation in many disciplines, including viral infections, gene modification, tumor diagnosis, gene therapy, and cellular distribution of rare mRNA copies. PCR/RT-PCR in situ: Light and Electron Microscopy covers methods of in situ polymerase chain reaction (PCR) and reverse transcription PCR (RT-PCR), two new approaches in visualizing very low amounts of DNA and RNA in tissues and cell cultures at the light and electron microscopy levels. Written by experts in this field, the book provides theoretical consideration, as well as practical approaches to in situ PCR. The authors provide detailed protocols for each step, including the preparation of tissue samples, the rationale for the design of primers and revelation. They also emphasize the need for appropriate controls to meet the requirements of in situ PCR and RT-PCR specificity. Organized in a user-friendly two-column format, this book will provide you with tools necessary to perform and optimize these sensitive and powerful techniques in your research protocols.

About the Author

Gérard Morel, Ph.D., D.Sc., is a research director at the National Center of Scientific Research (CNRS), at University Claude Bernard-Lyon 1, Villeurbanne, France. Mireille Raccurt, Ph.D., is an engineer in biology in a laboratory of the CNRS (Molecular Physiology), at Claude Bernard-Lyon 1 University, Villeurbanne, France.