PRINS and In Situ PCR Protocols

GOSDEN, J.R.:

ISBN 10: 0896033953 / ISBN 13: 9780896033955

Published by CHAPMAN & HALL LTD., 1996

About this Item

178 pp., numerous partly coloured figs., ring binding The techniques of oligonucleotide-primed in situ synthesis (PRINS), and in situ PCR have come of age. These techniques offer accurate and cost-effective diagnostic methods. The techniques are developing so rapidly that this set of protocols is intended t Sprache: Deutsch.

Seller Inventory # 17904

Bibliographic Details

• Title: PRINS and In Situ PCR Protocols
• Publisher: CHAPMAN & HALL LTD.
• Publication Date: 1996
• Language: German
• ISBN 10: 0896033953
• ISBN 13: 9780896033955
• Binding: 07
• Edition: 1997.
• Condition: Wie neu
• Seller catalogs:
  • Varia

About this title

Synopsis

Cutting edge researchers demonstrate step-by-step how oligonucleotide primers may be successfully used to detect and amplify or extend complimentary sequences in situ. Through these procedures-often invented by the authors-the door is opened to rapid identification and characterization of chromosomal DNA sequences, viral genomes, and rare messenger RNAs in cells-at hitherto unmatched degrees of sensitivity and specificity. Their innovative techniques-suitable for both novice and experienced researchers-have rapidly become indispensable for many clinical diagnostic procedures, whether in quantification of chromosomes in the identification of aneuploidy for prenatal diagnosis, or in the identification of viral infection in the early stages, or of rare messenger RNAs present in cells.

Review

...[a] splendid little book which covers the field quite well...Chapetr 16, which is a general review of in situ PCR written by Aidan Long and Paul Komminoth...is by far the best review of the approach...yet seen and should be mandatory reading for anyone intending to try in situ PCR. -FEBS Letters

"...useful to all those increasingly applying such techniques to in situ analysis...this book contains clear practical instructions that are easy to follow, and is well illustrated. It is essential for any laboratory contemplating exploiting this powerful technique."-SGM Quarterly