Parentage Analysis And Breed Characterization Of Dogs: DNA Testing In Dogs

Muhammad Sajid Tahir, Maria Arshad, Khalid Abdul Majeed

ISBN 10: 3847312189 / ISBN 13: 9783847312185

Published by LAP LAMBERT Academic Publishing 2011-12-19, 2011

About this Item

Seller Inventory # 6666-IUK-9783847312185

Bibliographic Details

• Title: Parentage Analysis And Breed Characterization Of Dogs: DNA Testing In Dogs
• Publisher: LAP LAMBERT Academic Publishing 2011-12-19
• Publication Date: 2011
• Language: English
• ISBN 10: 3847312189
• ISBN 13: 9783847312185
• Binding: Paperback
• Condition: New

About this title

Synopsis

Pakistan has vast population of dogs belonging to different breeds.Most of the dogs have no pedigree record which is a great threat to conservation of different breeds.No study on DNA fingerprinting of dogs has been conducted in Pakistan.DNA fingerprinting of dogs is necessary to overcome the problems like forensic cases,individual identity in case of fertilization by more than one male and ownership disputes.Microsatellite markers have been proved as an efficient tool for parentage testing and breed characterization of dogs. In this study,a panel of microsatellite markers was developed.Blood samples were taken from cephalic vein of two breeds of dogs(German shepherd and Labrador retriever). DNA was extracted and primers were optimized.Multiplex PCR was performed and genotyping was done for the PCR products on non denaturing polyacrylamide gel.These results were analyzed statistically.Almost all of the microsatellite markers showed significant variations in both breeds.Results of this study lead to development of a panel of microsatellite markers which can be used for parentage analysis and breed characterization of dogs.

About the Author

Mr. Muhammad Sajid Tahir, a famous celebrity in his home, was born in his home town Narang Mandi, Pakistan. After showing sparkling performance in his education with average marks but good concepts, he graduated as Doctor of Veterinary Medicine, completed M.Phil in Molecular Biology & Biotechnology from UVAS, Lahore and wrote this book finally.