A Physicochemical Perspective On Evolutionarily Related Repeated, Interspaced, Palindromic Sequence-Associated Effectors For Editing Genetic Material
Language: English
Published by Hussaini Begum, 2025
- Softcover
- New
Seller: PBShop.store UK, Fairford, GLOS, United KingdomPBShop.store UK
AbeBooks seller since June 11, 1999
Condition: New
US$ 38.55
Quantity: Over 20 available
Add to basketItem description from seller
New Book. Shipped from UK. Established seller since 2000.
Seller Inventory # L2-9798230131236
- Title
- A Physicochemical Perspective On Evolutionarily Related Repeated, Interspaced, Palindromic Sequence-Associated Effectors For Editing Genetic Material
- Author
- Leon, Lucia
- Publisher
- Hussaini Begum
- Publication year
- 2025
- Condition
- New
- Binding
- PAP
- Language
- English
- ISBN 13
- 9798230131236
- Item weight
- 263 grams
The CRISPR/Cas9 system is a naturally occurring, adaptive heritable immune system which defends invading phage attack and present in the whooping numbers of prokaryotes.This has been repurposed as a powerful tool which is guided by RNA and targets DNA for genome engineering which works by making site-specific DNA break and its subsequent repair by cellular machinery. The simplicity and multiplexity of the CRISPR system make it more sought after than other older counterparts viz. ZFNs and TALENs, and this has led to harnessing of CRISPR in gene therapy programmable gene regulation, cellular imagings, synthetic biology and nucleic acid detection among others. The CRISPR genome editing system includes an endonuclease, Cas9 derived from type II CRISPR-Cas system and a chimeric single-guide RNA (sgRNA). Cas9 and sgRNA forms ribonucleoprotein (RNP) binary complex and upon recognition of a protospacer adjacent motif (PAM) it binds to the substrate DNA, and forms ternary complex by unwinding the PAM upstream region with subsequent formation of R-loop. Successful R-loop formation displaces PAM- containing DNA strand and results in formation of a heteroduplex only when the target DNA strand finds complementary to sgRNA sequence. It is worth mentioning Cas9 remains inactive inthe absence of sgRNA and sgRNA binding induces structural changes in Cas9 from apo toactive form.
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PBShop.store UK
Fairford, GLOS, United Kingdom
AbeBooks seller since June 11, 1999
Shipping rates from United Kingdom to U.S.A.
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Hardbacks, PaperbacksSeller's business information
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