The Plant Cytoskeleton Methods and Protocols

Quantification of Microtubule Bundling Activity of MAPs Using TIRF Microscopy.- Actin: Static and Dynamic Studies.- 3D Visualization of Microtubules in Epidermal Pavement Cells.- Quantitative Analysis of Microtubule Organization in Leaf Epidermis Pavement Cells.- Single Cell Confinement Methods to Study Plant Cytoskeleton.- Documentation of Microtubule Collisions with Myosin VIII ATM1 Containing Membrane-Associated Structures.- Imaging the Plant Cytoskeleton by High-Pressure Freezing and Electron Tomography.- Confocal Microscopic Assays of Mitotically Active Proteins in an Agrobacterial Infiltration-Based, Cell Division-Enabled Leaf System of Tobacco (Nicotiana benthamiana).- Assessment of Spindle Shape Control by Spindle Poleward Flux Measurements and FRAP Bulk Analysis.- Expansion Microscopy of Plant Cells (PlantExM).- Microfluidic Device for High-Resolution Cytoskeleton Imaging and Wash Out Assays in Physcomitrium (Physcomitrella) patens.- Using Spinning Disk Microscopy to Observe the Mitotic and Cytokinetic Apparatus in Physcomitrium patens.- Gaining Insight into Large Gene Families with the Aid of Bioinformatic Tools.- Cell-to-Cell Connectivity Assays for the Analysis of Cytoskeletal and Other Regulators of Plasmodesmata.- Studying Nuclear Dynamics in Response to Actin Disruption In Planta.- Cytoskeleton Remodelling in Arabidopsis Stigmatic Cells Following Pollination.- Investigation of ROP GTPase Activity and Cytoskeleton Dynamics during Tip Growth in Root Hairs and Pollen Tubes.- Functional Analysis of Phospholipid Signaling and Actin Dynamics: The Use of Apical Growing Tobacco Pollen Tubes in a Case Study.- Microtubule Reorganization during ABA-Induced Stomatal Closure in Arabidopsis.- Imaging of Cortical Microtubules in Plants under Salt Stress.- Analysis of Actin Array Rearrangement during the Plant Response to Bacterial Stimuli.- Live-Cell Imaging of Cytoskeletal Responses and Trafficking during Fungal Elicitation.- Visualization and Quantification of the Dynamics of Actin Filaments in Arabidopsis Pollen Tubes.- Non-Invasive Long-Term Imaging of the Cytoskeleton in Arabidopsis Seedlings.- Visualization of Cytoskeleton Organization and Dynamics in Elongating Cotton Fibers by Live-Cell Imaging.- Methods to Visualize and Quantify Cortical Microtubule Arrays in Arabidopsis Conical Cells.- Studying the Organization of the Actin Cytoskeleton in the Multicellular Trichomes of Tomato.- Light Microscopy Technologies and the Plant Cytoskeleton.- Investigating Plant Protein-Protein Interactions Using FRET-FLIM with a Focus on the Actin Cytoskeleton. Sprache: Englisch Gewicht in Gramm: 854.…