Plant DNA Infectious Agents

Josef Schell

ISBN 10: 3709174589 / ISBN 13: 9783709174586

Published by Springer, 2011

About this Item

Druck auf Anfrage Neuware - Printed after ordering - There has been recent rapid progress in the transformation of plants with foreign DNA, making use either of the natural routes of genetic invasion that viruses and bacteria have developed, or of chemical, mechanical and electrical tricks to make plant protoplast membranes permeable to nucleic acids. Genes integrated into plant virus genomes can be carried systemi cally from the initial site of infection into the rest of the plant. Genes placed between the borders of Agrobacterium tumefaciens T-DNA can be transferred into single cells or plant tissue, which then divides to produce wound calli, or as in the case of an Agrobacterium rhizogenes infection, grow out into new roots. Calli and roots can be grown into whole plants. If virus genomes are placed between the T-DNA borders, a very effective infectious route, termed 'agroinfection', is established. Once inside a pro toplast, DNA finds its way into the nucleus where it can finally integrate into the resident chromosome and be expressed. Whether it can also find its way into chloroplasts is not yet clear, but at least translation products can be targeted into this organelle. Regeneration of whole organisms from single cells is a special feature of plants and offers a unique tool to study genes in a multicellular organism. In addition, as in animal cells, transcription and translation of trans forming genes can be studied in plant cells during 'transient expression'.

Seller Inventory # 9783709174586

Bibliographic Details

• Title: Plant DNA Infectious Agents
• Publisher: Springer
• Publication Date: 2011
• Language: English
• ISBN 10: 3709174589
• ISBN 13: 9783709174586
• Binding: Taschenbuch
• Condition: Neu
• Dimensions: 254W x 178H x 20D millimeters 254 millimeters width by 178 millimeters height by 20 millimeters depth
• Weight: 692 grams

About this title

Synopsis

There has been recent rapid progress in the transformation of plants with foreign DNA, making use either of the natural routes of genetic invasion that viruses and bacteria have developed, or of chemical, mechanical and electrical tricks to make plant protoplast membranes permeable to nucleic acids. Genes integrated into plant virus genomes can be carried systemi­ cally from the initial site of infection into the rest of the plant. Genes placed between the borders of Agrobacterium tumefaciens T-DNA can be transferred into single cells or plant tissue, which then divides to produce wound calli, or as in the case of an Agrobacterium rhizogenes infection, grow out into new roots. Calli and roots can be grown into whole plants. If virus genomes are placed between the T-DNA borders, a very effective infectious route, termed "agroinfection", is established. Once inside a pro­ toplast, DNA finds its way into the nucleus where it can finally integrate into the resident chromosome and be expressed. Whether it can also find its way into chloroplasts is not yet clear, but at least translation products can be targeted into this organelle. Regeneration of whole organisms from single cells is a special feature of plants and offers a unique tool to study genes in a multicellular organism. In addition, as in animal cells, transcription and translation of trans­ forming genes can be studied in plant cells during "transient expression".