This practical guide details the essential methodologies for the isolation, identification, and quantification of Influenza A viruses, specifically focusing on the H1N1pdm09 strain using MDCK cell culture systems. It provides a comprehensive workflow that includes standardized protocols for the maintenance and subculturing of MDCK cells, viral propagation techniques, and molecular confirmation via RT-PCR targeting the HA gene. Furthermore, the text offers in-depth instruction on viral quantification methods, employing Hemagglutination (HA) assays to determine total viral titers and TCID50 assays using the Reed-Muench method for calculating infectious titers. By combining theoretical background with rigorous experimental data and step-by-step procedural flowcharts, this work serves as a vital resource for researchers and laboratory professionals establishing robust platforms for influenza virus characterization and antiviral drug discovery.

"About this title" may belong to another edition of this title.

Store Description

We specialise in General Interest Books from South Asian countries.

Visit Seller's Storefront

Seller's business information

BOOKS AND PERIODICALS AGENCY LTD
90 Barnet Gate Lane, Barnet, EN5 2AX, United Kingdom

Sale & Shipping Terms

Terms of Sale

Returns accepted if you are not satisfied with the Service or Book.

Shipping Terms

Best packaging and fast delivery

Shipping rates from United Kingdom to U.S.A.

Shipping rates from United Kingdom to U.S.A.
Order quantity	14 to 45 business days	5 to 10 business days
First item	US$ 8.67	US$ 13.14

Delivery times are set by sellers and vary by carrier and location. Orders passing through Customs may face delays and buyers are responsible for any associated duties or fees. Sellers may contact you regarding additional charges to cover any increased costs to ship your items.