A Practical Guide to the Study of Calcium in Living Cells describes popular techniques along with helpful do's and don't's and computer programs. The volume enables investigators to evaluate confocal images, use the latest dyes, and design Calcium buffers appropriate to their research needs. This book is designed for laboratory use by graduate students, technicians, and researchers in many disciplines, ranging from molecular to cellular levels of investigation.

Describes techniques for detection of [Ca2+]I: Ca2+ - sensitive microelectrodes
Fluorescent dyes
Luminescent proteins
Includes techniques for perturbing intracellular Ca2+
Covers detailed methodology plus problems and pitfalls of each technique
Contains a practical guide to preparing Ca2+ buffers with an easy-to-use computer program
Color plates illustrate techniques such as
Confocal ratio-imaging
Use of aequorin

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University of California, Santa Barbara, USA

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