Practical Handbook on Veterinary Biochemistry and Biotechnology

Contents: 1. Units of Measurement and Strength of Solution. 2. Preparation and Standardization of Acids and Alkalies. 3. Preparation of Buffers and Determination of pH. 4. Titration Curve of Acid Versus Base. 5. Physical Properties of Carbohydrates. 6. Reactions of Monosaccharides and Their Identification. 7. Reactions of Disaccharides and Their Identification. 8. Preparation of Osazone. 9. Reactions of Polysaccharides and Their Identification. 10. Estimation of Lactose in Milk. 11. Determination of Acid Number of Fat/Oil. 12. Colour Reactions of Proteins. 13. Precipitation Reactions of Proteins. 14. Identification of the Lipids. 15. Estimation of Amino Acids (Sorensen’s Method). 16. Effect of pH and Temperature on Enzyme Activity. 17. Collection of Blood and Separation of Plasma and Serum. 18. Estimation of Blood Glucose. 19. Estimation of Serum Total Proteins. 20. Electrophoretic Separation of Proteins. 21. Estimation of Serum Urea. 22. Estimation of Serum Cholesterol. 23. Estimation of Serum Bilirubin. 24. Estimation of Serum Creatinine. 25 Estimation of Serum Uric Acid. 26. Estimation of Electrolytes & Other Metabolic Intermediates of Blood. 27. Physical Examination of Urine. 28. Microscopic Examination of Urine. 29. Normal Constituents of Urine. 30. Abnormal (Pathological) Constituents of Urine. 31. Isolation of Genomic DNA from Mammalian Cells. 32. Isolation of Genomic DNA from Bacteria. 33. Isolation of Plasmid DNA from Bacterial Cells. 34. Isolation of Total RNA from Mammalian Cells/Tissue. 35. Quantification and Purity Analysis of Nucleic Acids. 36. Polymerase Chain Reaction. 37. Real Time PCR. 38. Agarose Gel Electrophoresis. 39. Directional Cloning of Target Gene(s) in Plasmid Vector(s). 40. Extraction of Total Proteins Using SDS. 41. Analysis of Total Proteins by SDS-PAGE. 42. Bioinformatics and the Databases. 43. Sequence Alignment by Database Similarity Searching. 44. Primer Designing. 45. ELISA: Principle, Protocol and Applications. The "Practical Handbook on Veterinary Biochemistry and Biotechnology" covers the analytical techniques commonly used in Veterinary Biochemistry and Biotechnology and can be used as a bench top guide for undergraduate as well as post-graduate students of veterinary and animal sciences. The practical exercises covered in this book contain basic principles and working protocol for the techniques. The principal goal of the book is to provide students with full details of the practical steps necessary for carrying out each practical successfully by providing knowledge about the chemistry behind it. The book will enable the students and the researchers to develop a sound understanding of the principle and methodology of the biochemical and biotechnological techniques required to carry out basic and applied research in the subject. The topics have been selected following the VCI-MSVE syllabus, 2016 for Veterinary Biochemistry and some common Biotechnological techniques have also been included. In addition, descriptions about a few important Bioinformatics tools like sequence alignment and primer designing, etc. are also included to give an initial idea about these tools to the students.…