Cover has edge wear, creases, minor scratches, small bent/rubbed cover/page corners, rubbed/slightly chipped spine. Owner's name marked out on half-title page. No writing in text.

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Bibliographic Details

Title

Protein Engineering: A Practical Approach

Publisher

Irl Pr, McLean, Virginia, U.S.A.

Publication Date

1993

Language

English

ISBN 10

0199631387

ISBN 13

9780199631384

Binding

Soft cover

Condition

Very Good

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About this title

Synopsis

Protein engineering is the rational modification or redesign of proteins using genetic engineering. Thus, it is now possible to modify enzyme specifics, remodel antibodies, and redesign many multi-domain proteins for therapeutic purposes. While the procedures for the introduction of mutations have become routine, predicting and understanding the effects of these mutations can be complicated. This volume provides a comprehensive guide to the methods used at every stage of the engineering process, from the choice of mutation strategy, through protein stability studies, to critical evaluations of mammalian, yeast, and bacterial host expression systems. Protein Engineering: A Practical Approach is the first practical guide to this fascinating mixture of molecular biology, protein structure analysis, computation, and biochemistry. It combines a thorough theoretical foundation with detailed protocols and will be invaluable to all research workers in the area, from graduate students to senior investigators.

Review

'the first practical guide to this fascinating area of science ... the major parts of the book are very well written and of great didactic value ... The book is recommended for undergraduate and graduate students and to researchers in the field of protein engineering.' Arvind M. Kayastha, Biochemical Education 21(4) 1993 'The problem is to produce a specific purified protein in significant quantities. This book provides some practical details that can help in this task.' International Journal of Biochemistry, Vol. 25, No. 12, 1993 'Protein Engineering: A Practical Approach is the first reference book in this network of molecular biology, structure of proteins and biochemistry. It combines theretical background and detailed procedures; both together enable researchers in this field to plan and carry out experiments for their own projects. This book should therefore serve as the standard text for students and scientists in the field of protein engineering.' Arzneimittel-Forschung/Drug Research Issue 12/93

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