Protein Methods: Second Edition

Daniel M. Bollag

ISBN 10: 0471118370 / ISBN 13: 9780471118374

Published by Wiley-Liss, 1996

About this Item

Leichte Rillen / Abschurfungen / Risse / Knicke. This revised and expanded Second Edition remains a comprehensive source for proven protein techniques, now divided into two parts. It begins with essential chapters from the first edition, updated to reflect significant methodological advancements. Notably, the chapter on protein isolation includes a new section on isolating proteins from inclusion bodies. The second section introduces four new chapters focused on protein purification and crystallization.Chapters cover a range of topics, including preparation for protein isolation, extraction and solubilization, concentration determination, and gel electrophoresis under various conditions. Each chapter includes detailed information on required reagents and equipment, "view-at-a-glance" tabs for easy navigation, and illustrations for gel applications and other methods. Additional features include a complete supplier list, appendices with basic chemical parameters and molecular data, key references, descriptive tables, and safety notes.Reviews of the first edition praised its clarity and practicality, highlighting its usefulness for both beginners and experienced scientists. The book is designed to provide readers with the necessary information to perform techniques effectively, minimizing the need for additional reference sources.

Seller Inventory # eb97472f-045b-4f99-9a4e-b45962fd22c6

Bibliographic Details

• Title: Protein Methods: Second Edition
• Publisher: Wiley-Liss
• Publication Date: 1996
• Language: English
• ISBN 10: 0471118370
• ISBN 13: 9780471118374
• Binding: Softcover
• Edition: 2nd Edition
• Condition: Fine

About this title

Synopsis

Protein Methods
Daniel M. Bollag/ Michael D. Rozycki /Stuart J. Edelstein
Reviews from the first edition
"...it is very well written...would certainly be of use to undergraduate and postgraduate students entering the complex world of protein preparation and to more experienced scientists entering this field for the first time."-Biotechnology and Applied Biochemistry
"The book presents these topics with loads of practical detail, so that the reader has little need to consult other reference sources to carry out the techniques described. All in all, a useful book..." -Theoretical & Applied Genetics
"...clearly written with protocols that are easy to follow and the text is well spread-out and easy to read...all methods are fully referenced...a useful book for beginners at a reasonable price." -FEBS Letters
This revised and expanded Second Edition of Protein Methods remains the first source for a complete summary of tested and proven protein techniques. Now divided into two parts, the book begins with the essential chapters from the first edition, updated to reflect important changes in methodology. The chapter on protein isolation includes a new section focusing on the isolation of proteins from inclusion bodies. In the second section, four new chapters are devoted to protein purification and crystallization.
Chapters include
* Preparation for Protein Isolation
* Protein Extraction and Solubilization
* Protein Concentration Determination
* Concentrating Protein Solutions
* Gel Electrophoresis Under Denaturing Conditions
* Gel Electrophoresis Under
...and feature
* reagent items and equipment required for each method
* chapter "view-at-a-glance" tabs
* illustrations for all gel applications and other methods
* complete supplier list
* appendices of basic chemical parameters and molecular data
* key references
* descriptive tables
* Safety notes.

About the Author

Daniel M. Bollag and Michael D. Rozycki are the authors of Protein Methods, 2nd Edition, published by Wiley.