Proteins are an integral part of molecular and cellular structure and function and are probably the most purified type of biological molecule. In order to elucidate the structure and function of any protein it is first necessary to purify it. Protein purification techniques have evolved over the past ten years with improvements in equipment control, automation, and separation materials, and the introduction of new techniques such as affinity membranes and expanded beds. These developments have reduced the workload involved in protein purification, but there is still a need to consider how unit operations linked together to form a purification strategy, which can be scaled up if necessary. The two Practical Approach books on protein purification have therefore been thoroughly updated and rewritten where necessary. The core of both books is the provision of detailed practical guidelines aimed particularly at laboratory scale purification. Information on scale-up considerations is given where appropriate. The books are not comprehensive but do cover the major laboratory techniques and common sources of protein. Protein Purification Techniques focuses on unit operations and analytical techniques. It starts with an overview of purification strategy and then covers initial extraction and clarification techniques. The rest of the book concentrates on different purification methods with the emphasis being on chromatography. The final chapter considers general scale-up considerations. Protein Purification Applications describes purification strategies from common sources: mammalian cell culture, microbial cell culture, milk, animal tissue, and plant tissue. It also includes chapters on purification of inclusion bodies, fusion proteins, and purification for crystallography. A purification strategy that can produce a highly pure single protein from a crude mixture of proteins, carbohydrates, lipids, and cell debris to is a work of art to be admired. These books (available individually or as a set)are designed to give the laboratory worker the information needed to undertake the challenge of designing such a strategy.

About the Author

Simon Roe is at AEA Technology Products and Systems, Biosciences, Harwell.

"About this title" may belong to another edition of this title.

Store Description

Visit Seller's Storefront

Seller's business information

Book Booth
OH, U.S.A.

Sale & Shipping Terms

Terms of Sale

Checks and money orders are also accepted; please make checks or money orders payable to
Carol Casciani
441 W. Bagley Rd.
#173
Berea OH 44017

International orders must be pay...

More Information

Shipping Terms

Payment accepted via credit card, check/money order, or PayPal. Credit cards accepted via ABEbooks or PayPal ONLY -- please do not send us your credit card information, as we have no way of processing it ourselves. Our PayPal account is bookbooth@usa.com. If paying via PayPal, please remember to include shipping & handling charges. Checks and money orders are also accepted; please make them payable to: C.M. Casciani, 441 W. Bagley Rd. #173, Berea OH 44017. Library and institutional orders welcome; please email us if you require any special handling. International orders must be payable in US dollars. SHIPPING & HANDLING: all books shipped in USA via USPS; Media Mail 7-14 business days for delivery, $4.00 for first book - $1.50 each additional book; or, Priority Mail 2-4 business days for delivery, $6.00 first book - $2.50 each additional book. Returns for reasonable grading of condition; email us prior to return for shipping instructions.

Shipping rates within U.S.A.

Shipping rates within U.S.A.
Order quantity	5 to 14 business days	3 to 6 business days
First item	US$ 5.90	US$ 12.70

Delivery times are set by sellers and vary by carrier and location. Orders passing through Customs may face delays and buyers are responsible for any associated duties or fees. Sellers may contact you regarding additional charges to cover any increased costs to ship your items.