Based on a popular course at Cold Spring Harbor Laboratory, this new manual assembles cutting–edge protocols, helpful hints, and lecture notes to teach researchers from a wide variety of disciplines the essential methods of proteomics using state–of–the–art instrumentation. Detailed protocols involving protein microarrays, liquid chromatography, high–throughput cloning of expression constructs, IMAC, mass spectrometry, MALDI–TOF, and MudPIT are provided, along with well–illustrated descriptions of experimental procedures and lists of recommended Web sites and reading material. Proteomics: A Cold Spring Harbor Laboratory Course Manual can be used both as the basis for a course and as a detailed bench manual for those performing indispensable proteomic experiments. It is authored by Andrew J. Link and Joshua LaBaer, both leaders in their fields, who bring complementary expertise to the manual.

Review

[T]he book is very well presented, written in a logical and concise way, and unequivocally solid in terms of scientific content, with many ready-to-use, robust procedures tested and validated over the years by recognized experts. I warmly recommend this volume to anyone who wants to implement proteomics techniques in the laboratory, or who simply wishes to get a practical sense of these techniques, useful in correctly interpreting the significance of proteomic data in the scientific literature. The Quarterly Review of Biology --The Quarterly Review of Biology

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