Here are methods for studying five types of clinically-relevant mammalian stems cells: mammary, nerve, skeletal muscle, endothelial and mesenchymal. Covers obtaining, characterizing and studying these cells or adapting them to the stem cells of choice. Editor(s): Conboy, Irina M.; Schaffer, David V.; Barcellos-Hoff, Mary Helen; Li, Song. Series: Methods in Molecular Biology. Num Pages: 207 pages, 8 black & white tables, biography. BIC Classification: PSAK; PSF. Category: (P) Professional & Vocational. Dimension: 258 x 186 x 20. Weight in Grams: 638. . 2010. 2010th Edition. hardcover. . . . . Books ship from the US and Ireland.

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Bibliographic Details

Title: Protocols for Adult Stem Cells
Publisher: Humana
Publication Date: 2010
Language: English
ISBN 10: 1607610620
ISBN 13: 9781607610625
Binding: Hardcover
Condition: New

About this title

Synopsis

Although embryonic stem cells currently enjoy the public limelight and show great pr- ise for cell based medical therapies, it is the adult stem cells which are responsible for the body’s natural ability to fght disease, heal and recover, or fail and succumb to various maladies. The study of mammalian adult stem cells has surged recently, most likely from a maturation of stem cell studies in the classical developmental model organisms and in hematopoeisis. All the tissues of the body examined so far are generated and regenerated from stem cells, it has been an important frst step to adapt or devise new methods to identify and obtain these cells in quantity and purity for further study. Culture techniques have been optimized for managing the growth and differentiation of stem cells in vitro; as some stem cells are pluripotent, often the method is to guide the fate of such cells among the possible differentiation fates. Much of this work, and that in the classical model org- isms, has helped defne the aspects of the stem cell environment or niche that are crucial for both growth and differentiation, and these studies have moved in vivo at increasingly higher resolution. Importantly, the in vivo niche is a current target for bioengineering the matrix and signaling factors. Herein, we present methods for studying six types of mammalian stem cells, m- mary, neural, mesenchymal, endothelial, dendritic, and muscle.

About the Author

Irina M. Conboy University of California, Berkeley, CA
David V. Schaffer University of California, Berkeley, CA
Mary Helen Barcellos-Hoff Lawrence Berkeley National Laboratory, Berkeley, CA
Song Li University of California, Berkeley, CA

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