Protocols for Neural Cell Culture

In Protocols for Neural Cell Culture, 2nd Ed., Sergey Fedoroff and Arleen Richardson extensively revise and expand their best-selling and highly praised collection of popular neural tissue culture protocols. This second edition of unfailingly reproducible methods includes key techniques to study the development and differentiation of the nervous system, new methods for isolating and propagating progenitor cells that can be stimulated to differentiate into either neurons or various types of glial cells, and timely procedures for eliminating contaminating neural or nonneural cells. Important new procedures for the transfection of cells in culture with nontoxic mutant herpes simplex virus are also included, as are essential procedures for quantification, growth assays, evaluating neuroactive agents, preparing neural material for electron microscope analysis, and dissection.

"In addition to covering standard protocols in well-established culture systems, this is one of the first manuals to include a chapter on protocols to culture neural stem cells. Another unique feature is the inclusion of pictures to facilitate the dissection of particular brain areas, which will be of great help for workers not completely familiar with different aspects of developmental neuroanatomy. . .This is a valuable tool for "culturists" of neural cells. This edition updates protocols for the culturing of neural cell types traditionally used in laboratories studying different aspects of neurobiology and it provides easy-to follow instructions for setting up and maintaining cultures of progenitor cells that will be used in many laboratories in years to come."-Doody's Health Science Book review Journal

"...presents a series of excellent neural tissue culture protocols designed for bench use."
-Trends in Cell Biology

"...a valuable resource that will find its place on many laboratory shelves..."
-Trends in Biotechnology

"...the recipes are comprehensive and easy to follow."
-Trends in Neurosciences

"...indispensable for every project in neuroscience."
-Doody's Health Sciences Book Review Journal