Title: Protocols for Nucleic Acid Analysis by Nonradioactive Probes [Methods in Molecular Biology, Vol. 28]
Publisher: Humana Press, Totowa, N.J.
Publication Date: 1994
Language: English
ISBN 10: 089603254X
ISBN 13: 9780896032545
Binding: Paperback
Condition: New

About this title

Synopsis

This book offers tried and tested protocols for analyzing nucleic acids without the use of radioactive probes. It also covers Southern (DNA) blot and Northern (RNA) blotting protocols in detail, from how to isolate good quality nucleic acids, through the use of the widest range of probe systems involving digoxigenin, biotin, fluorescein, horseradish peroxidase labelling, and colorimetric and chemiluminescent detection. Also included are novel techniques such as RAPD, reverse dot blot, and nucleic acid sequence-based amplification.

Review

...this book provides a collection of good protocols which are in general clearly written. They can be easily employed on the bench without being a specialist in a particular fields. - FEBS Letters

...Covers the basics of a variety of probe-labelling systems very well...a very interesting read. It could save you money and trouble from the radiation safety officer.-Biotechnology and Applied Biochemistry

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