Quantifying cell-ECM adhesion by single cell force spectroscopy | Quantifying adhesive interactions between cells and extracellular matrix by atomic force microscopy based single cell force spectroscopy

Anna Taubenberger

ISBN 10: 3838124278 / ISBN 13: 9783838124278

Published by Südwestdeutscher Verlag für Hochschulschriften, 2015

About this Item

Quantifying cell-ECM adhesion by single cell force spectroscopy | Quantifying adhesive interactions between cells and extracellular matrix by atomic force microscopy based single cell force spectroscopy | Anna Taubenberger | Taschenbuch | 184 S. | Englisch | 2015 | Südwestdeutscher Verlag für Hochschulschriften | EAN 9783838124278 | Verantwortliche Person für die EU: preigu GmbH & Co. KG, Lengericher Landstr. 19, 49078 Osnabrück, mail[at]preigu[dot]de | Anbieter: preigu.

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Bibliographic Details

• Title: Quantifying cell-ECM adhesion by single cell force spectroscopy | Quantifying adhesive interactions between cells and extracellular matrix by atomic force microscopy based single cell force spectroscopy
• Publisher: Südwestdeutscher Verlag für Hochschulschriften
• Publication Date: 2015
• Language: English
• ISBN 10: 3838124278
• ISBN 13: 9783838124278
• Binding: Taschenbuch
• Condition: Neu
• Dimensions: 220W x 150H x 12D millimeters 220 millimeters width by 150 millimeters height by 12 millimeters depth
• Weight: 292 grams
• Seller catalogs:
  • Bücher

About this title

Synopsis

Integrin-mediated adhesive interactions of cells with the extracellular matrix (ECM) regulate important cellular functions and play a critical role in disease. Therefore, studying the mechanisms underlying integrin-binding and regulation is of great interest. In the presented work three respective biological questions have been addressed using Atomic Force Microscopy based Single Cell Force Spectroscopy (AFM-SCFS). The work is split into six chapters. The first chapter provides an introduction into cell-ECM interactions. Thereafter different adhesion assays are compared and contrasted, and the theoretical Bell-Evans model is discussed. In chapter four, alpha2beta1-integrin mediated cell adhesion to collagen I is quantified. Thereafter the effects of the exposure of cryptic integrin binding sites within collagen I on pre-osteoblasts are analysed. At last, the role of BCR/ABL in modulating myeloid progenitor cell adhesion to stromal cells and ECM-coated substrates is studied. Taken together, the work demonstrates that AFM-SCFS is a versatile tool that permits monitoring of cell adhesion from single-molecule recognition events to the formation of more complex adhesion sites.

About the Author

Anna Taubenberger, born 1980 in Kulmbach, Germany, graduated 2004 from the TU Dresden with a degree in Engineering. Between 2005 and 2009 she did her PhD at the Biotechnological Centre Dresden on the presented work. Since 2009 she is working as a DFG postdoctoral research fellow at Queensland University of Technology in Brisbane, Australia.