Title: Quantitation of mRNA by Polymerase Chain Reaction: Nonradioactive PCR Methods (Springer Lab Manuals)
Publisher: Springer
Publication Date: 2011
Language: English
ISBN 10: 3642797148
ISBN 13: 9783642797149
Binding: Soft cover
Condition: New

About this title

Synopsis

In this laboratoy "cook-book", the authors provide a concise guide to PCR-based quantification of nucleic acids using exclusivley nonradioactive detection. The procedures described represent state-of-the-art know-how and are presented in a very detailed and practice-oriented manner. Thus, this laboratory guide enables professionals and beginners to adopt easily quantitative PCR protocols into their own research.

From the Back Cover

In this laboratory "cook-book", the authors provide a concise guide to PCR-based techniques for quantifying nucleic acids in biological and clincial samples using exclusively nonradioactive detection methods, e.g. HPLC, biotin and digoxigenin based protocols. Each method presentation also includes sections on theory, reagents, standards, applicability, limitations, and trouble shooting. In addition to the protocols, the authors also provide the necessary information on: general aspects of nucleic acid quantitation; design of PCR standards; mRNA purification; cDNA synthesis; solution hybridization; DNA sequencing. This laboratory guide enables professionals as well as beginners to adopt easily quanatitative PCR protocols into their own clinical or biomedical research.

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