Quantitation of mRNA by Polymerase Chain Reaction/Nonradioactive PCR Methods

Köhler, Thomas; Laßner, Dirk; Rost, Anne-Katrin; Thamm, Barbara; Pustowoit, Barbara; Remke, Harald

ISBN 10: 3540591923 / ISBN 13: 9783540591924

Published by Springer, 1995

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Bibliographic Details

• Title: Quantitation of mRNA by Polymerase Chain Reaction/Nonradioactive PCR Methods
• Publisher: Springer
• Publication Date: 1995
• Language: English
• ISBN 10: 3540591923
• ISBN 13: 9783540591924
• Binding: Hardcover
• Condition: New

About this title

Synopsis

In this laboratory "cook-book", the authors provide a concise guide to PCR-based techniques to quantify nucleic acids in biological and clinical samples using exclusively nonradioactive detection methods, e.g. HPLC, biotin and digoxigenin based protocols. Each method presentation also includes sections on theory, reagents, standards, applicability, limitations, and trouble shooting. In addition to the protocols, the authors also provide the necessary information on: general aspects of nucleic acid quantitation; design of PCR standards; mRNA purification; cDNA synthesis; solution hybridization; DNA sequencing. This laboratory guide enables professionals as well as beginners to adopt easily quantitative PCR protocols into their own clinical or biomedical research.

From the Back Cover

In this laboratory "cook-book", the authors provide a concise guide to PCR-based techniques for quantifying nucleic acids in biological and clincial samples using exclusively nonradioactive detection methods, e.g. HPLC, biotin and digoxigenin based protocols. Each method presentation also includes sections on theory, reagents, standards, applicability, limitations, and trouble shooting. In addition to the protocols, the authors also provide the necessary information on: general aspects of nucleic acid quantitation; design of PCR standards; mRNA purification; cDNA synthesis; solution hybridization; DNA sequencing. This laboratory guide enables professionals as well as beginners to adopt easily quanatitative PCR protocols into their own clinical or biomedical research.