Title: Quantitative DNA method in forensic DNA laboratory: Screening tool for efficient genotyping
Publisher: LAP LAMBERT Academic Publishing
Publication Date: 2013
Language: English
ISBN 10: 3659415804
ISBN 13: 9783659415807
Binding: Soft cover
Condition: New

About this title

Synopsis

Quantification of human DNA in forensic samples is an important step during STR profiling because the STR genotyping is sensitive to the quantity of DNA used in the PCR reaction.This study focuses on the importance of quantification in the entire process of genetic analysis.Two real time PCR platforms were used to compare two commercial kits in terms of DNA quantification.It was found out that accuracy of absolute quantification values in commercial quantification kits is strongly dependent on the construction of calibration curve. Especially low template DNA samples were used to assess whether QuantifilerTM or Plexor� HY System can determinate a minimum quantification value(cut off value)below which STR profiles would consistently fail to be detected. The usage of Plexor� HY System enabled to determine the cut off quantification value more exactly probably due to different molecular background and chemistry used in this kit.Reliability and other issues connected with cut off value are discussed. In order to better understand the relationship between the quantity of DNA and the number of detectable loci series the dilution expexperiment with standard DNA007 was done.

About the Author

Ivana Koljensic graduated from Charles University in Prague with a specialization anthropology and human genetics. During the study have focused on forensic genetics. She is a member of the international student organizations. And sees her future in forensic sciences.

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StainesBookHub aim to offer our customers a variety of the old & latest Books. With the Experience of more than 15 Years, we always keep an eye on the latest trends in Books and put our customers' wishes first, we try our best to give books to our customers on lowest prices. We ship Books through Fedex Priority. That is why we have satisfied customers all over the world, and are thrilled to be a part of the Books industry. StainesBooksHub also sell books in Bulk, We try our best to Provied High ...

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