RNA Detection : Methods and Protocols

ISBN 10: 1493984187 / ISBN 13: 9781493984183

Published by Humana Press, 2018

About this Item

Seller Inventory # 26376474008

Bibliographic Details

• Title: RNA Detection : Methods and Protocols
• Publisher: Humana Press
• Publication Date: 2018
• Language: English
• ISBN 10: 1493984187
• ISBN 13: 9781493984183
• Binding: Soft cover
• Condition: New

About this title

Synopsis

This volume introduces different concepts and methods of detecting RNA in biological material in a variety of model systems. The chapters in this book discuss methods that will answer numerous biological questions that arise in the study of RNAs. Some of the topics covered in this book are single mRNA molecule detection in embryos and neurons; detection of mRNA and associated molecules by ISH-IEM on frozen sections; optimizing molecular beacons for intracellular analysis of RNA; imaging translation dynamics of single mRNA molecules in live cells; preparation of high-throughput sequencing libraries; and capturing RNA binding proteins in embryos and in cell-culture. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, RNA Detection: Methods and Protocols is a valuable resource for novel and experiences scientist in the expanding field of RNAs.