RNA Processing: A Practical ApproachVolume II (The ^APractical Approach Series)

ISBN 10: 019963470X / ISBN 13: 9780199634705

Published by Oxford University Press, 1994

About this Item

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Bibliographic Details

• Title: RNA Processing: A Practical ApproachVolume II (The ^APractical Approach Series)
• Publisher: Oxford University Press
• Publication Date: 1994
• Language: English
• ISBN 10: 019963470X
• ISBN 13: 9780199634705
• Binding: Soft cover
• Condition: New

About this title

Synopsis

As post-transcriptional events become increasingly recognized as critical regulation sites of gene expression in eukaryotic cells, research interest in RNA processing has grown more and more intense. RNA Processing: A Practical Approach, available in two volumes, offers detailed guidance on all major aspects of the subject. Step-by-step protocols from leading laboratories are presented for studying the termination, end-processing, capping, methylation, splicing, and editing of mRNA. Protocols designed for the study of mRNA stability and processing of rRNA and tRNA are included as well, with full descriptions of the synthesis and purification of RNA substrate for in vitro work, the characterization of specific RNAs, and the isolation and analysis of ribonucleoprotein complexes. Taken together, the two volumes are invaluable laboratory companions to researchers working in mRNA, rRNA, and tRNA expression. The contributors' lucid explanatory style and comprehensive coverage will be welcomed by both experienced researchers and students embarking on such studies for the first time.

About the Author

Stephen J. Higgins and B. David Hames are both at the University of Leeds.