Real-Time PCR : Advanced Technologies and Applications

Nick A Saunders

ISBN 10: 1908230223 / ISBN 13: 9781908230225

Published by Caister Academic Press Limited Jul 2013, 2013

About this Item

Neuware - Real-time PCR technology is an established powerful research tool used in many scientific disciplines and is also utilized for mainstream testing in the regulated markets, such as food, veterinary, and human in-vitro diagnostics. This essential manual provides both the novice and experienced user with an invaluable reference to a wide-range of real-time PCR technologies and applications, and it provides an overview of the theory of this increasingly important technique. Renowned international authors present detailed technical insights into the underlying principles, methods, and practice of real-time PCR. The initial chapters cover the important aspects of real-time PCR, including choosing an instrument and probe system, set-up, nucleic acid synthesis, sample extraction controls, and validation and data analysis. Further chapters provide a comprehensive overview of important real-time PCR methodologies, such as quantification, expression analysis, and mutation detection. This is complemented by the final chapters, which address the application of real-time PCR to diagnosis of infectious diseases, biodefense, veterinary science, food authenticity, and molecular haplotyping. This timely and authoritative volume serves both as a basic introduction to real-time PCR and as a source of current trends and applications for those already familiar with the technology. The editors also aim to stimulate readers of all levels to develop their own innovative approaches to real-time PCR. The book will be an essential text for all laboratories using PCR.

Seller Inventory # 9781908230225

Bibliographic Details

• Title: Real-Time PCR : Advanced Technologies and Applications
• Publisher: Caister Academic Press Limited Jul 2013
• Publication Date: 2013
• Language: English
• ISBN 10: 1908230223
• ISBN 13: 9781908230225
• Binding: Buch
• Condition: Neu
• Dimensions: 240W x 161H x 24D millimeters 240 millimeters width by 161 millimeters height by 24 millimeters depth
• Weight: 842 grams

About this title

Synopsis

Real-time PCR technology is an established powerful research tool used in many scientific disciplines and is also utilized for mainstream testing in the regulated markets, such as food, veterinary, and human in-vitro diagnostics. This essential manual provides both the novice and experienced user with an invaluable reference to a wide-range of real-time PCR technologies and applications, and it provides an overview of the theory of this increasingly important technique. Renowned international authors present detailed technical insights into the underlying principles, methods, and practice of real-time PCR. The initial chapters cover the important aspects of real-time PCR, including choosing an instrument and probe system, set-up, nucleic acid synthesis, sample extraction controls, and validation and data analysis. Further chapters provide a comprehensive overview of important real-time PCR methodologies, such as quantification, expression analysis, and mutation detection. This is complemented by the final chapters, which address the application of real-time PCR to diagnosis of infectious diseases, biodefense, veterinary science, food authenticity, and molecular haplotyping. This timely and authoritative volume serves both as a basic introduction to real-time PCR and as a source of current trends and applications for those already familiar with the technology. The editors also aim to stimulate readers of all levels to develop their own innovative approaches to real-time PCR. The book will be an essential text for all laboratories using PCR.

About the Author

Martin A. Lee is the author of four books, including most recently "Smoke Signals: A Social History of Marijuana" "Medical, Recreational and Scientific". He is the cofounder of the media watch group FAIR and the director of Project CBD, a medical science information service. He is also the author of "Acid Dreams" and "The Beast Reawakens", and his writing has appeared in many publications, including "The Washington Post", "The Los Angeles Times", "Harper s Magazine", "Le Monde Diplomatique", "Rolling Stone", "The Nation", Salon.com, HuffingtonPost.com, and TheDailyBeast.com.