Recombinant DNA Methodology II (Selected Methods in Enzymology)

The critically acclaimed laboratory standard for forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerlyawaited, frequently consulted, and praised by researchers and reviewers alike. More than 250 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences.

* Methods for:
* DNA isolation and cloning
* Synthesizing complementary DNA (cDNA)
* Cleaving and manipulating DNA
* Selecting useful reporter genes
* Constructing vectors for cloning genes
* Constructing expression vectors
* Site-directed mutagenesis and gene disruption
* Identifying and mapping genes
* Transforming animal and plant cells
* Sequencing DNA
* Amplifying and manipulating DNA and PCR
* Detecting DNA - protein interaction

The 'Methods in Enzymology' series represents the gold-standard.
--NEUROSCIENCE
Incomparably useful.
--ANALYTICAL BIOCHEMISTRY
It is a true 'methods series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page.
--BIO/TECHNOLOGY
The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection.
--CHEMISTRY IN INDUSTRY
The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced.
--AMERICAN SOCIETY OF MICROBIOLOGY NEWS
If we had some way to find the work most often consulted in the laboratory, it could well be Colowick and Kaplans multivolume series Methods in Enzymology...a great work.
--ENZYMOLOGIA
A series that has established itself as a definitive reference for biochemists.
--JOURNAL OF CHROMATOGRAPHY