Restriction Endonucleases

Pingoud, Alfred (Edited by)

ISBN 10: 3540205020 / ISBN 13: 9783540205029

Published by Springer, 2004

About this Item

2004 edition. 443 pages. German language. 9.25x6.25x1.25 inches. In Stock.

Seller Inventory # x-3540205020

Bibliographic Details

• Title: Restriction Endonucleases
• Publisher: Springer
• Publication Date: 2004
• Language: English
• ISBN 10: 3540205020
• ISBN 13: 9783540205029
• Binding: Hardcover
• Condition: Brand New
• Weight: 0.98 kilograms

About this title

Synopsis

Restriction enzymes are highly specific nucleases which occur ubiquitously among prokaryotic organisms, where they serve to protect bacterial cells against foreign DNA. Many different types of restriction enzymes are known, among them multi-subunit enzymes which depend on ATP or GTP hydrolysis for target site location. The best known representatives, the orthodox type II restriction endonucleases, are homodimers which recognize palindromic sequences, 4 to 8 base pairs in length, and cleave the DNA within or immediately adjacent to the recognition site. In addition to their important biological role (up to 10 % of the genomes of prokaryotic organisms code for restriction/modification systems!), they are among the most important enzymes used for the analysis and recombination of DNA. In addition, they are model systems for the study of protein-nucleic acids interactions and, because of their ubiquitous occurence, also for the understanding of the mechanisms of evolution.

From the Back Cover

Restriction enzymes are highly specific nucleases which occur ubiquitously among prokaryotic organisms, where they serve to protect bacterial cells against foreign DNA. Many different types of restriction enzymes are known, among them multi-subunit enzymes which depend on ATP or GTP hydrolysis for target site location. The best known representatives, the orthodox type II restriction endonucleases, are homodimers which recognize palindromic sequences, 4 to 8 base pairs in length, and cleave the DNA within or immediately adjacent to the recognition site. In addition to their important biological role (up to 10 % of the genomes of prokaryotic organisms code for restriction/modification systems!), they are among the most important enzymes used for the analysis and recombination of DNA. In addition, they are model systems for the study of protein-nucleic acids interactions and, because of their ubiquitous occurence, also for the understanding of the mechanisms of evolution.

The present book deals with all aspects of restriction endonucleases including nomenclature, diversity, evolution, genetics, structure and function, mechanism of target site location and DNA recognition, enzymology, protein design, and provides a description of the history of the discovery of and the research on restriction enzymes.