As post-translational events become increasingly recognized as critical regulation sites of gene expression in eukaryotic cells, intense research interest in RNA processing has rapidly grown. RNA Processing: A Practical Approach, available in two volumes, offers detailed guidance on all major aspects of the subject. Step-by-step protocols from leading laboratories are presented for studying the termination, end-processing, capping, methylation, splicing, and editing of mRNA. Protocols designed for the study of mRNA stability and processing of rRNA and tRNA are included as well, with full descriptions of the synthesis and purification of RNA substrate for in vitro work, the characterization of specific RNAs, and the isolation and analysis of ribonucleoprotein complexes. Taken together, the two volumes are invaluable laboratory companions to researchers working in mRNA, rRNA, and tRNA expression. The contributors' lucid explanatory style and comprehensive coverage will be welcomed by both experienced researchers and by students embarking on such studies for the first time.

About the Author

Stephen J. Higgins and B. David Hames are both at the University of Leeds.

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