Screening for Reverse Cholesterol Transport efficiency

Language: English

Published by Scholars' Press, 2014

3639664035 / 9783639664034

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nach der Bestellung gedruckt Neuware - Printed after ordering - Generation of foam cells, an essential step for reverse cholesterol transport (RCT) studies, uses the technique of receptor dependent macrophage loading with radiolabeled acetylated Low Density Lipoprotein (Ac-LDL). In this study, we used the ability of a biologically relevant detergent molecule, Lysophosphatidylcholine (Lyso PtdCho), to form mixed micelles with cholesterol or cholesteryl ester (CE) to generate macrophage foam cells. Fluorescent or radiolabelled cholesterol / Lyso PtdCho mixed micelles were prepared and incubated with RAW 264.7 or mouse peritoneal macrophages. Results showed that such micelles were quite stable at 4°C and retained the solubilized cholesterol during one month storage. Macrophages incubated with cholesterol or CE (unlabeled, fluorescently labeled or radiolabeled) / Lyso PtdCho mixed micelles accumulated CE as documented by microscopy, lipid staining, labeled oleate incorporation, and by thin layer chromatography (TLC). Such foam cells unloaded cholesterol when incubated with high density lipoprotein (HDL) and not with oxidized HDL (Ox-HDL). We propose that stable cholesterol or CE / Lyso PtdCho micelles would offer advantages over existing methods. O.

Seller Inventory # 9783639664034

Title
Screening for Reverse Cholesterol Transport efficiency
Author
Bhaswati Sengupta
Publisher
Scholars' Press
Publication year
2014
Condition
Neu
Binding
Taschenbuch
Language
English
ISBN 10
3639664035
ISBN 13
9783639664034
Item weight
161 grams
Dimensions
220x150x6 mm

AHA-BUCH GmbH

Einbeck, Germany

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