Summaraizes methods for successfully using sequence analysis software packages. This work shows how to examine the data produced by automated sequencers, how to assess its quality, how to remove extraneous data, how to align multiple overlapping sequence fragments for either assembly into sequence contigs or comparison with similar sequences. Series: Methods in Molecular Biology. Num Pages: 324 pages, biography. BIC Classification: PDN; PSF; UY. Category: (P) Professional & Vocational; (UP) Postgraduate, Research & Scholarly; (UU) Undergraduate. Dimension: 229 x 148 x 20. Weight in Grams: 653. . 1996. Hardback. . . . .

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Bibliographic Details

Title: Sequence Data Analysis Guidebook
Publisher: Humana Press Inc.
Publication Date: 1996
Language: English
ISBN 10: 0896033589
ISBN 13: 9780896033580
Binding: Hardcover
Condition: New

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Synopsis

Computers have revolutionized the analysis of sequencing data. It is unlikely that any sequencing projects have been performed in the last few years without the aid of computers. Recently their role has taken a further major step forward. Computers have become smaller and more powerful and the software has become simpler to use as it has grown in sophistication. This book reflects that change since the majority of packages described here are designed to be used on desktop computers. Computer software is now available that can run gels, collect data, and assess its accuracy. It can assemble, align, or compare multiple fragments, perform restriction analyses, identify coding regions and specific motifs, and even design the primers needed to extend the sequencing. Much of this soft� ware may now be used on relatively inexpensive computers. It is now possible to progress from isolate d DNA to database submission without writing a single base down. To reflect this progression, the chapters in our Sequence Data Analysis Guidebook are arranged, not by software package, but by fimction. The early chapters deal with examining the data produced by modem automated sequenc� ers, assessing its quality, and removing extraneous data. The following chap� ters describe the process of aligning multiple sequences in order to assemble overlapping fragments into sequence contigs to compare similar sequences from different sources. Subsequent chapters describe procedures for compar� ing the newly derived sequence to the massive amounts of information in the sequence databases.

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