Single Cell Diagnostics | Methods and Protocols

Alan R Thornhill

ISBN 10: 161737654X / ISBN 13: 9781617376542

Published by Humana Press, 2010

About this Item

Single Cell Diagnostics | Methods and Protocols | Alan R Thornhill | Taschenbuch | xi | Englisch | 2010 | Humana Press | EAN 9781617376542 | Verantwortliche Person für die EU: Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin, juergen[dot]hartmann[at]springer[dot]com | Anbieter: preigu.

Seller Inventory # 107146436

Bibliographic Details

• Title: Single Cell Diagnostics | Methods and Protocols
• Publisher: Humana Press
• Publication Date: 2010
• Language: English
• ISBN 10: 161737654X
• ISBN 13: 9781617376542
• Binding: Taschenbuch
• Condition: Neu
• Dimensions: 229W x 152H x 11D millimeters 229 millimeters width by 152 millimeters height by 11 millimeters depth
• Weight: 295 grams
• Seller catalogs:
  • Bücher

About this title

Synopsis

The aim of Single Cell Diagnostics: Methods and Protocols is for all readers to extend their knowledge and expertise in analysis of single cells. The book starts with laser assisted cell collection, non-invasive assessment of single cells and moves through the techniques of standard fluorescence in situ hybridization and polymerase chain reaction (PCR) As the reader moves through the book, the scope and complexity of each technique gradually increases as real-time quantitative PCR, isothermal whole genome amplification, comparative genomic hybridization, real-time gene expression analysis and the production of RNA and cDNA libraries are covered. The book closes with the application of customized microarrays to the study of single cells.

Review

From the reviews:

"Single cell diagnostics has become an increasingly important field for both clinicians and researchers who are involved in infertility treatment. ... For researchers this book provides not only detailed state of the art protocols for single cell diagnostics, but also valuable notes on troubleshooting and pitfalls. For clinicians in the field of infertility this nice overview gives an insight into single cell diagnostics and its scientific background. ... This book can be warmly recommended to clinicians and researchers caring for patients with fertility problems." (G. Haidl and J. P. Allam, Andrologia, Vol. 40 (1), 2008)

"This book details innovative methods for analyzing gene expression, chromosome organization, and proteins in single cells and cell extracts. ... The book is written for basic science and clinical researchers, particularly those providing care to couples seeking advice regarding infertility and inherited genetic disorders. ... The chapters are written by experts and the book is carefully edited. ... The laboratory protocols and tips will be valued by laboratory scientists interested in forensic medicine, reproductive medicine, and developmental genetics." (Bruce A. Fenderson, Doody’s Review Service, October, 2007)