Single Nucleotide Polymorphisms: Methods and Protocols (Methods in Molecular Biology, Vol. 212)

Pui-Yan Kwok

ISBN 10: 0896039684 / ISBN 13: 9780896039681

Published by Humana Press, 2002

About this Item

USED book in GOOD condition. Great binding, pages and cover show normal signs of wear from use.

Seller Inventory # GCM.1AZX

Bibliographic Details

• Title: Single Nucleotide Polymorphisms: Methods and Protocols (Methods in Molecular Biology, Vol. 212)
• Publisher: Humana Press
• Publication Date: 2002
• Language: English
• ISBN 10: 0896039684
• ISBN 13: 9780896039681
• Binding: Hardcover
• Condition: good

About this title

Synopsis

Pui-Yan Kwok, MD, PhD, has assembled a comprehensive collection of readily reproducible techniques for the difficult process of single nucleotide polymorphisms (SNP) discovery and genotyping. These cutting-edge protocols for mutation/SNP detection utilize denaturing high-performance liquid chromatography (dHPLC), single-strand conformation polymorphism (SSCP), conformation-sensitive gel electrophoresis (CSGE), chemical cleavage, and direct sequencing. Equally powerful and up-to-date methods are given for genotyping SNPs, including molecular beacons, the Taqman assay, single-base extension approaches, pyrosequencing, ligation, the Invader assay, and primer extension with mass spectrometry detection.