Software-assisted Method Development In High Performance Liquid Chromatography

This handbook gives a general overview of the possibilities in recent developments in chromatographic retention modeling. As a result of the latest developments in modeling software, several new features are now accessible, opening a new level in HPLC method development.

Many of these current possibilities in software assisted liquid chromatographic method modeling for analytical purposes are presented. Several modes of chromatography, including Reversed-Phase Liquid Chromatography (RPLC), Ion Exchange Chromatography (IEX), Hydrophobic Interaction Chromatography (HIC), and Hydrophilic Interaction Liquid Chromatography (HILIC) are explained in detail. For all these chromatographic modes, the most important variables for tuning retention and selectivity are exposed.

Beside the industrial and practical benefits of retention modeling, the possibilities in teaching and education are also illustrated. Finally, numerous representative industrial examples are shown, to highlight the benefits, time and cost savings offered by state-of-the-art software assisted HPLC method development.

Readership: Recommended for analysts, chromatographers, chemists, chemical engineers who are working in pharmaceutical, bioanalytical, medical laboratories. Also recommended for teachers working at universities for the education of analytical chemistry, liquid chromatography, design of experiments, optimization processes analysts, chromatographers, chemists, chemical engineers, students in analytical chemistry and non-experts interested in software based optimization and modeling.