Title: Somatic Cell Nuclear Transfer in Goat (Capra hircus): Production of Cloned Embryos by Hand-made Cloning Method
Publisher: LAP LAMBERT Academic Publishing 2012-03-14
Publication Date: 2012
Language: English
ISBN 10: 3848426544
ISBN 13: 9783848426546
Binding: Paperback
Condition: New

About this title

Synopsis

Nuclear transfer is a very effective method for propagation of valuable, extinct and endangered animals. Hand-made cloning (HMC) is an effective alternative to the conventional micromanipulator based technique in some domestic species. The present study was carried out for the establishment of an effective enucleation method, for selection of suitable somatic cell as nuclear donor and for development of an optimum culture system for in vitro culture of zona free cloned embryos of goat (Capra hircus). An efficient chemically assisted enucleation procedure was established for goat HMC. Treatment of donor cells with roscovitine resulted in better hand-made cloned blastocyst yield. Production of hand-made cloned goat embryos was better with electric pulse activation than Ca ionophore activation. A highly effective culture system for the development of hand-made cloned goat embryos was established. Furthermore, fetal fibroblast cells proved to be better nuclear donor than fresh cumulus cells or cultured cumulus cells.

About the Author

Yogesh S. Akshey, Arun Kumar De and Dhruba Malakar:Currently working on in vitro and in vivo production of embryos and their transfer to recipient animals, stem cells, somatic cell nuclear transfer, induced pluripotent stem cells and regulation of oocyte competence in farm animals. Published a number of research articles in reputed journals.

"About this title" may belong to another edition of this title.

Store Description

Visit Seller's Storefront

Seller's business information

WRAP Ltd
Unit 4, 119 Loverock Rd, Reading, RG30 1DZ, United Kingdom

Sale & Shipping Terms

Terms of Sale

TBA

Shipping Terms

Shipping costs are based on books weighing 2.2 LB, or 1 KG. If your book order is heavy or oversized, we may contact you to let you know extra shipping is required.

Shipping rates from United Kingdom to U.S.A.

Shipping rates from United Kingdom to U.S.A.
Order quantity	14 to 21 business days	14 to 21 business days
First item	US$ 20.80	US$ 20.80

Delivery times are set by sellers and vary by carrier and location. Orders passing through Customs may face delays and buyers are responsible for any associated duties or fees. Sellers may contact you regarding additional charges to cover any increased costs to ship your items.