Nuclear transfer is a very effective method for propagation of valuable, extinct and endangered animals. Hand-made cloning (HMC) is an effective alternative to the conventional micromanipulator based technique in some domestic species. The present study was carried out for the establishment of an effective enucleation method, for selection of suitable somatic cell as nuclear donor and for development of an optimum culture system for in vitro culture of zona free cloned embryos of goat (Capra hircus). An efficient chemically assisted enucleation procedure was established for goat HMC. Treatment of donor cells with roscovitine resulted in better hand-made cloned blastocyst yield. Production of hand-made cloned goat embryos was better with electric pulse activation than Ca ionophore activation. A highly effective culture system for the development of hand-made cloned goat embryos was established. Furthermore, fetal fibroblast cells proved to be better nuclear donor than fresh cumulus cells or cultured cumulus cells.

About the Author

Yogesh S. Akshey, Arun Kumar De and Dhruba Malakar:Currently working on in vitro and in vivo production of embryos and their transfer to recipient animals, stem cells, somatic cell nuclear transfer, induced pluripotent stem cells and regulation of oocyte competence in farm animals. Published a number of research articles in reputed journals.

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