Stability Indicating RP-HPLC Method For Combined Dosage Form: Of Nebivolol HCl and Indapamide

Imran Chaki

ISBN 10: 3659908010 / ISBN 13: 9783659908019

Published by LAP LAMBERT Academic Publishing, 2016

About this Item

136 pages. 8.66x5.91x0.31 inches. In Stock.

Seller Inventory # 3659908010

Bibliographic Details

• Title: Stability Indicating RP-HPLC Method For Combined Dosage Form: Of Nebivolol HCl and Indapamide
• Publisher: LAP LAMBERT Academic Publishing
• Publication Date: 2016
• Language: English
• ISBN 10: 3659908010
• ISBN 13: 9783659908019
• Binding: Paperback
• Condition: Brand New
• Weight: 0.25 kilograms

About this title

Synopsis

A simple, rapid, accurate, sensitive, precise, and isocratic RP- HPLC method was develop for analysis of Nebivolol Hydrochloride and Indapamide in combined tablet dosage form. The best separation was achieved by C-18 reversed phase column (250 mm x 4.6 mm, 5μm particle) with 50 mM Potassium dihydrogen phosphate, pH 3.5 adjusted with ortho phosphoric acid, acetonitrile and triethylamine in ratio (30:70:2 v/v) as mobile phase. Flow rate was set 1 ml/min and detection was carried out at 254 nm. Retention time of Nebivolol Hydrochloride and Indapamide was found to be 3.407 min. and 5.470 min. respectively. The Method has been validated for the combination with parameters like linearity, accuracy and precision and rubustness. Linear response for Nebivolol Hydrochloride and Indapamide were in the range of 50-150 μg/ml and 15-45 μg/ml respectively. The Percentage recoveries obtained for Nebivolol Hydrochloride and Indapamide were found to be 99.87 ± 0.20 and 99.93 ± 0.187 respectively. The study further extended to develop stability indicating method where acid, base, oxidation and thermal degradation was performed.

About the Author

Imran A. Chaki, Master of Pharmacy, Pharmaceutical Quality Assurance at Gujarat Technological University. Research Associate at Quest Care in Quality Assurance Department.