Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) : Methods and Protocols

Bettina Warscheid

ISBN 10: 1493911414 / ISBN 13: 9781493911417

Published by Springer New York, Springer New York, 2014

About this Item

Druck auf Anfrage Neuware - Printed after ordering - Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC): Methods and Protocols provides a synopsis of a large array of different SILAC methods by presenting a set of protocols that have been established by renowned scientists and their working groups. These include methods and protocols for the labeling of various model organisms as well as advanced strategies relying on SILAC, e.g. for the analysis of protein interactions, the mapping of posttranslational modifications or the characterization of subcellular proteomes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC): Methods and Protocols will serve students and experienced scientists alike as a valuable reference of how to make use of the SILAC technology for their own research.

Seller Inventory # 9781493911417

Bibliographic Details

• Title: Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) : Methods and Protocols
• Publisher: Springer New York, Springer New York
• Publication Date: 2014
• Language: English
• ISBN 10: 1493911414
• ISBN 13: 9781493911417
• Binding: Buch
• Condition: Neu
• Dimensions: 260W x 183H x 27D millimeters 260 millimeters width by 183 millimeters height by 27 millimeters depth
• Weight: 925 grams

About this title

Synopsis

Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC): Methods and Protocols provides a synopsis of a large array of different SILAC methods by presenting a set of protocols that have been established by renowned scientists and their working groups. These include methods and protocols for the labeling of various model organisms as well as advanced strategies relying on SILAC, e.g. for the analysis of protein interactions, the mapping of posttranslational modifications or the characterization of subcellular proteomes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC): Methods and Protocols will serve students and experienced scientists alike as a valuable reference of how to make use of the SILAC technology for their own research.