Stimulated Raman Scattering Microscopy: Techniques and Applications

Stimulated Raman Scattering Microscopy: Techniques and Applications describes innovations in instrumentation, data science, chemical probe development, and various applications enabled by a state-of-the-art stimulated Raman scattering (SRS) microscope. Beginning by introducing the history of SRS, this book is composed of seven parts in depth including instrumentation strategies that have pushed the physical limits of SRS microscopy, vibrational probes (which increased the SRS imaging functionality), data science methods, and recent efforts in miniaturization.

This rapidly growing field needs a comprehensive resource that brings together the current knowledge on the topic, and this book does just that. Researchers who need to know the requirements for all aspects of the instrumentation as well as the requirements of different imaging applications (such as different types of biological tissue) will benefit enormously from the examples of successful demonstrations of SRS imaging in the book.

Led by Editor-in-Chief Ji-Xin Cheng, a pioneer in coherent Raman scattering microscopy, the editorial team has brought together various experts on each aspect of SRS imaging from around the world to provide an authoritative guide to this increasingly important imaging technique. This book is a comprehensive reference for researchers, faculty, postdoctoral researchers, and engineers.

• Includes every aspect from theoretic reviews of SRS spectroscopy to innovations in instrumentation and current applications of SRS microscopy
• Provides copious visual elements that illustrate key information, such as SRS images of various biological samples and instrument diagrams and schematics
• Edited by leading experts of SRS microscopy, with each chapter written by experts in their given topics

Dr. Ji-Xin Cheng attended University of Science and Technology of China (USTC) from 1989 to 1994. He carried out his PhD study on bond-selective chemistry at USTC. As a graduate student, he worked as a research assistant at Universite Paris-sud on vibrational spectroscopy and the Hong Kong University of Science and Technology (HKUST) on quantum dynamics theory. After postdoctoral training on ultrafast spectroscopy at HKUST, he joined Sunney Xie’s group at Harvard University as a postdoc and worked on the development of CARS microscopy. Cheng joined Purdue University as Assistant Professor in 2003, promoted to Associate Professor in 2009 and Full Professor in 2013. He joined Boston University as the Inaugural Theodore Moustakas Chair Professor in Photonics and Optoelectronics in 2017. For his pioneering contributions to the chemical imaging field, Cheng received the 2020 Pittsburg Spectroscopy Award, the 2019 Ellis R. Lippincott Award, and the 2015 Craver Award.

Dr. Wei Min graduated from Peking University in 2003. He received his Ph.D. from Harvard University in 2008 studying single-molecule biophysics with Prof. Sunney Xie. After continuing his postdoctoral work in the Xie group, Dr. Min joined the faculty at Columbia University in 2010 and was promoted to Full Professor there in 2017. Dr. Min’s current research interests focus on developing novel optical spectroscopy and microscopy technology to address biomedical problems. His group has made important contributions to the development of stimulated Raman scattering (SRS) microscopy and its broad application in biomedical imaging. Dr. Min’s contribution has been recognized by a number of honors, including Scientific Achievement Award from Royal Microscopical Society (2021), Pittsburgh Conference Achievement Award (2019), Coblentz Award of Molecular Spectroscopy (2017), ACS Early Career Award in Experimental Physical Chemistry (2017), and NIH Director’s New Innovator Award (2012).

Dr. Yasuyuki Ozeki received B.S., M.S. and Dr. Eng. Degrees in Electronic Engineering from the University of Tokyo, Tokyo, Japan, in 1999, 2001, and 2004, respectively. In 2004, he joined Furukawa Electric Co., Ltd., as a postdoctoral researcher of Japan Science and Technology Agency (JST). In 2006, he joined Department of Material and Life Science, Osaka University, Osaka, Japan, as an assistant professor. From 2009 to 2013, he was also PRESTO researcher of JST. In 2013, he was appointed as an Associate Professor of Department of Electrical Engineering and Information Systems, the University of Tokyo, Tokyo, Japan, and was promoted to a Full Professor in 2021. His work covers millimeter-wave photonics, nonlinear fiber optics, ultrafast lasers, and their application to microprocessing and biomedical microscopy. His current research focuses on biomedical imaging by stimulated Raman scattering (SRS) microscopy, and its related technologies including highly functional ultrafast laser sources, detection electronics, image processing, etc.

Dario Polli is Associate Professor of Physics at Politecnico di Milano (Italy) since 2014, where he is heading a research group of more than 10 people including post-docs, Ph.D. and diploma students. He is affiliated with the Center for Nano Science and Technology of the Italian Institute of Technology in Milan, Italy. His main research focus is on coherent Raman spectroscopy and microscopy, ultrafast and non-linear optics, Fourier-transform spectroscopy and time-resolved pump-probe spectroscopy and microscopy. He is the recipient of many research grants, including an ERC Consolidator grant on the development of high-speed broadband coherent Raman microscopy for fast and reliable tumour identification. He also devotes to technology transfer: he filed several patents and has founded two start-up companies in the field of photonics. Finally, he is passionate about Science divulgation to the public.