Study of retention mechanism and development of HILIC methods: Optimization of HILIC methods for determination of polar compounds in drug products using design of experiments approach

Nakov, Natalija

ISBN 10: 3659495301 / ISBN 13: 9783659495304

Published by LAP LAMBERT Academic Publishing, 2013

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Bibliographic Details

• Title: Study of retention mechanism and development of HILIC methods: Optimization of HILIC methods for determination of polar compounds in drug products using design of experiments approach
• Publisher: LAP LAMBERT Academic Publishing
• Publication Date: 2013
• Language: English
• ISBN 10: 3659495301
• ISBN 13: 9783659495304
• Binding: paperback
• Condition: New
• Book Type: book

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Synopsis

In the last twenty years hydrophilic interaction liquid chromatography (HILIC) has emerged as a new type of liquid chromatography for separation of polar compounds. In HILIC, a multimodal retention mechanism is present so it is very difficult to predict the impact of chromatographic parameters on the retention and separation of the compounds. Furthermore, the relationship between the pH of the mobile phase, the degree of ionization of the compounds, the surface of the stationary phase and the absorbed water layer is very complex, which significantly complicates the HILIC method development. In this book we experimentally determinate the contribution of partition, adsorption and ion exchange mechanism to the retention of several polar compounds on bare silica column. Also fast, simple and easily applicable HILIC methods for determination of polar compounds in different dosage forms were established using the univariate and design of experiments approach. The book could serve as a guide for the HILIC method development and optimization, and should be useful for practitioners working in analytical method development laboratories.

About the Author

M.Sci Natalija Nakov is an assistant and PhD Aneta Dimitrovska is a professor of Instrumental methods and Analytics of drugs at the Faculty of Pharmacy, University Ss Cyril and Methodius, Skopje, Republic of Macedonia. They have extensive experience in development of analytical methods for drug quality control.