TALENs

Ralf Kühn

ISBN 10: 1493929313 / ISBN 13: 9781493929313

Published by Springer New York Okt 2015, 2015

About this Item

This item is printed on demand - it takes 3-4 days longer - Neuware - This volume provides a comprehensive collection of protocols on new technology across various model organisms. Chapters describe species-specific methods to generate new mutants the content is completed by chapters on natural TAL effectors, TAL element DNA binding principles, TALEN target site prediction, and methods for the efficient construction of TALEN coding regions. While addition chapter focus on the application of TALEN as sequence-specific nucleases and TAL based gene activators or inhibitors and the visualization of chromatin dynamics in live cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, TALENs: Methods and Protocols aims to ensure successful results in the further study of this vital field. 288 pp. Englisch.

Seller Inventory # 9781493929313

Bibliographic Details

• Title: TALENs
• Publisher: Springer New York Okt 2015
• Publication Date: 2015
• Language: English
• ISBN 10: 1493929313
• ISBN 13: 9781493929313
• Binding: Buch
• Condition: Neu
• Dimensions: 260W x 183H x 21D millimeters 260 millimeters width by 183 millimeters height by 21 millimeters depth
• Weight: 808 grams

About this title

Synopsis

​This volume provides a comprehensive collection of protocols on new technology across various model organisms.  Chapters describe species-specific methods to generate new mutants the content is completed by chapters on natural TAL effectors, TAL element DNA binding principles, TALEN target site prediction, and methods for the efficient construction of TALEN coding regions. While addition chapter focus on the application of TALEN as sequence-specific nucleases and TAL based gene activators or inhibitors and the visualization of chromatin dynamics in live cells.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, TALENs: Methods and Protocols aims to ensure successful results in the further study of this vital field.