Three Phase Partitioning: Applications in Separation and Purification of Biological Molecules and Natural Products

Three Phase Partitioning: Applications in Separation and Purification of Biological Molecules and Natural Products presents applications in diverse areas of both chemical technology and biotechnology. This book serves as a single resource for learning about both the economical, facile and scalable processes, along with their potential for applications in the separation and purification of materials and compounds across the entire spectra of chemical and biological nature. The book begins by explaining the origins and fundamentals of TPP and continues with chapters on related applications, ranging from the purification of parasite recombinant proteases to oil extraction from oilseeds and oleaginous microbes, and more.

• Written by researchers who have been pioneers in developing and utilizing three phase partitioning
• Focuses on applications, with chapters detailing relevance to a wide variety of areas and numerous practical examples
• Designed to give laboratory workers the information needed to undertake the challenge of designing successful three-phase partitioning protocols

Dr. Munishwar Nath Gupta earned his PhD from Indian Institute of Science, Bengaluru, and completed post-doctoral positions at Massachusetts Institute of Technology (USA), University of Minnesota (USA), Lund University (Sweden), and University of Technology of Compiegne (France). He has taught chemistry, biochemistry, and biotechnology at Indian Institute of Technology, Delhi, between 1975-2016. He was awarded the National Science Talent fellowship (India) and Fellowships of National Academy of Sciences and Indian National Science Academy. He has edited three books on thermostability of enzymes, non-aqueous enzymology and affinity-based separation methods (published by Springer/Birkhauser). He was an Associate Editor of Biocatalysis and Biotransformation (Taylor and Francis) and founding and former editor-in-chief of Sustainable Chemical Processes (Springer). He’s served on editorial boards of several national and international journals and acted as a consultant to Novozyme (Denmark), Dabur (India), and other international companies. His research interests include applied biocatalysis and interfaces of biochemistry with nanotechnology.

Dr. Ipsita Roy earned her PhD from Indian Institute of Technology, Delhi, completed a post-doctoral position at Indian Institute of Technology, Delhi, and conducted an Alexander von Humboldt research fellowship at the University of Bonn (Germany). She has been teaching biotechnology and biochemistry at the postgraduate level at National Institute of Pharmaceutical Education and Research, S.A.S. Nagar, since 2005. Her research interests include stabilization of pharmaceutically important and disease-relevant proteins; aptamer technology; downstream processing of proteins.