Tissue Fixation and Processing

Uppala, Divya; Balla, Hyandavi; Pamidimukkala, Sahithi

ISBN 10: 6206740420 / ISBN 13: 9786206740421

Published by LAP LAMBERT Academic Publishing, 2025

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Bibliographic Details

• Title: Tissue Fixation and Processing
• Publisher: LAP LAMBERT Academic Publishing
• Publication Date: 2025
• Language: English
• ISBN 10: 6206740420
• ISBN 13: 9786206740421
• Binding: Soft cover
• Condition: New

About this title

Synopsis

This comprehensive manual provides an in-depth exploration of tissue fixation, processing, and embedding techniques essential in histopathology and biomedical research. Beginning with the fundamental concepts of autolysis and fixation, the book delves into the chemistry and classification of various fixatives including aldehydes, oxidizing agents, protein denaturants, and cross-linking compounds highlighting their mechanisms, applications, and impact on tissue morphology and staining properties. It further addresses the physical and chemical factors affecting fixation, such as pH, temperature, penetration rate, and osmolality. Detailed discussions cover both conventional and advanced methods like microwave fixation and the use of secondary fixatives for enhanced preservation. The book also provides extensive guidance on tissue processing stages, including dehydration, clearing, impregnation, and embedding using paraffin, celloidin, resins, and ester wax. Special sections include techniques for photomicrography, image analysis, and tumor marker identification, offering valuable insights for diagnostic and research applications.