Trinucleotide Repeats : Methods and Protocols

Guy-Franck Richard

ISBN 10: 1493997866 / ISBN 13: 9781493997862

Published by Springer New York, Springer US, 2020

About this Item

Druck auf Anfrage Neuware - Printed after ordering - This detailed book contains techniques to explore the unusual properties of the peculiar microsatellites known as trinucleotide repeats. Beginning with technical challenges raised by secondary structure-forming repeats and their propensity to contract and expand over time, the book continues with genetic screens in Saccharomycescerevisiae, experimental systems to study trinucleotide repeat instability in human cells, dedicated experimental systems in appropriate cell types, as well as gene therapy approaches using the CRISPR-Cas family of endonucleases. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Trinucleotide Repeats: Methods and Protocols serves as avaluable aid to experts and newcomers alike who seek to investigate this fascinating and ever-expanding field of study.

Seller Inventory # 9781493997862

Bibliographic Details

• Title: Trinucleotide Repeats : Methods and Protocols
• Publisher: Springer New York, Springer US
• Publication Date: 2020
• Language: English
• ISBN 10: 1493997866
• ISBN 13: 9781493997862
• Binding: Taschenbuch
• Condition: Neu
• Dimensions: 254W x 178H x 17D millimeters 254 millimeters width by 178 millimeters height by 17 millimeters depth
• Weight: 569 grams

About this title

Synopsis

This detailed book contains techniques to explore the unusual properties of the peculiar microsatellites known as trinucleotide repeats.  Beginning with technical challenges raised by secondary structure-forming repeats and their propensity to contract and expand over time, the book continues with genetic screens in Saccharomyces cerevisiae, experimental systems to study trinucleotide repeat instability in human cells, dedicated experimental systems in appropriate cell types, as well as gene therapy approaches using the CRISPR-Cas family of endonucleases.  Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Trinucleotide Repeats: Methods and Protocols serves as avaluable aid to experts and newcomers alike who seek to investigate this fascinating and ever-expanding field of study.