This work mainly highlights the novel source of urease enzyme and its application. Urease was obtained from the vegetable wastes i.e., pumpkin seeds and was purified and characterized and further immobilized in calcium alginate beads. The alginate immobilized enzyme was used for the detection of hazardous metals in aqueous solutions. This immobilized urease was also used for the blood urea estimation which compared favourably with those obtained by Autoanalyzer used in clinical pathology laboratory.

About the Author

Dr.Mahe Talat has done her Ph.D in Biochemistry(Enzyme Technology)from the Department of Biochemistry,Banaras Hindu University,Varanasi India in 2008.Presently a postdoctoral fellow in Nanoscience and Nanotech.Unit in the Department of Physics,BHU.VaranasiDr.Om Prakash is a Professor in the Department of Biochemistry Banaras Hindu University.

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