In Vitro Andrographolide Production From Irradiated Andrographis Sps.: Andrographolide Production Through Tissue Culture From The Callus

Kuruvilla, Susila, Pillai, P.R.Unnikrishna

ISBN 10: 3848432722 / ISBN 13: 9783848432721

Published by LAP LAMBERT Academic Publishing, 2012

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Bibliographic Details

• Title: In Vitro Andrographolide Production From Irradiated Andrographis Sps.: Andrographolide Production Through Tissue Culture From The Callus
• Publisher: LAP LAMBERT Academic Publishing
• Publication Date: 2012
• Language: English
• ISBN 10: 3848432722
• ISBN 13: 9783848432721
• Binding: Paperback
• Condition: Like New
• Book Type: book

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Synopsis

The effects of gamma irradiation on secondary metabolite production in callus cultures derived from four doses of gamma irradiated seedlings on Andrographis paniculata were studied. The effects of lower doses of gamma irradiation on secondary metabolite production in callus cultures were also examined in Andrographis echiodes, since the seedlings of these species were highly sensitive to higher doses of gamma irradiation. The results of this study indicated that gamma irradiation positively influencedandrographolide production in A.paniculata from the callus cultures. The significant impact of this study was that the callus cultures produced from non-irradiated seedlings showed the absence of andrographolide. The effects of four doses of gamma irradiation on the invitro production of andrographolide from the leaf explants of A.paniculata was studied. An enhanced rate of andrographolide was found in all the doses of samples using HPLC analysis after gamma irradiation. Gamma irradiation promoted the dipterpenoid production when the seedlings of A.echiodes were irradiated with 0.5kR of gamma rays. Further studies at the molecular and genomic level are required to know the exact mechan

About the Author

Dr.Susila Kuruvilla, Asso.Prof.of Botany,St.Joseph's College for Women, Alleppey-688003, Kerala, India.Area of Specialization-Plant Biotechnology.Dr.P.R.Unnikrishna Pillai, Asso.Prof.,Dept.of P.G.Studies & Research in Botany,S.D.College,Alleppey-688003,Kerala,India. Areas of Specialization-Cytogenetics, Plant Biotechnology.Produced 2 Ph.D.Degrees.