Yeast Hybrid Methods

Recognized as one of the most significant breakthroughs of molecular biology technology in the 1990s, the two-hybrid system provides a novel way of detecting protein-protein interactions through a powerful genetic approach. This approach has experienced rapid improvements and has been well adapted to various other purposes, such as detecting protein-DNA interactions (one hybrid) and RNA-protein interactions or multiple protein interactions (three-hybrid). Written by technical experts, this book covers essentially all these aspects as well as new developments in the field. Readers of this book are provided up-to-date detailed laboratory protocols as well as background on developmental history, basic technology, variations, special applications, troubleshooting suggestions, and important prospects for the future. There is also useful information on closely related technologies such as in vitro protein interaction detection methods, as well as both the bacterial and mammalian two-hybrid systems.