Cromeans (24 results)
- Softcover
Seller: GreatBookPrices, Columbia, MD, U.S.A.GreatBookPrices
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- Softcover
Seller: Grand Eagle Retail, Bensenville, IL, U.S.A.Grand Eagle Retail
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Paperback. Condition: new. Paperback. Enteroviruses, hepatitis A virus (HAV) and other enteric viruses can survive wastewater treatment processes, even after chlorination, and are found in the final effluents. These viruses can be detected by cell culture techniques with observations for cytopathic effect (CPE). Recently molecul…ar detection of viral nucleic acids has been used. Most viruses found in wastewater are RNA viruses and RT-PCR is a rapid and sensitive method to detect these single-strand RNA enteric viruses. This methodology does not distinguish between infectious and non-infectious viruses. Viruses inactivated in the treatment process can be detected but do not pose a public health threat. Methods are needed to quickly distinguish the infectious viruses from inactivated viruses, both of which may be present in effluents. In this project we investigated the use of a method that combines cell culture and molecular detection. If a sample contains viruses that replicate in cell culture even without CPE, the proof of replication can be demonstrated by the detection of a replicative form (RF) in cell culture that is only present during replication of infectious RNA viruses. Enteroviruses, hepatitis A virus (HAV) and other enteric viruses can survive wastewater treatment processes, even after chlorination, and are found in the final effluents. These viruses can be detected by cell culture techniques with observations for cytopathic effect (CPE). Recently molecular detection of viral nucleic acids has been used. Most viruses found in wastewater are RNA viruses and RT-PCR is a rapid and sensitive method to detect these single-strand RNA enteric viruses. This methodology does not distinguish between infectious and non-infectious viruses. Viruses inactivated in the treatment process can be detected but do not pose a public health threat. Methods are needed to quickly distinguish the infectious viruses from inactivated viruses, both of which may be present in effluents. In this project we investigated the use of a method that combines cell culture and molecular detection. If a sample contains viruses that replicate in cell culture even without CPE, the proof of replication can be demonstrated by the detection of a replicative form (RF) in cell culture that is only present during replication of infectious RNA viruses. A negative sense strand of RNA is generated from the viral positive strand virus, and these two are found primarily bound in a replicative form. This RNA was specifically detected by RT-PCR, including the positive and negative strands. This assay was developed and explored for the detection of low levels of CVB3 and HAV laboratory strains first and then for low levels of wild-type enteroviruses isolated from sewage samples. As few as four infectious units of wild-type enteroviruses, contained in 2.0 ml of water concentrate prepared from 600 ml of treated wastewater effluent, could be detected within two days. Treated wastewater effluents were collected weekly over an 18 months period, and viruses were concentrated with a developed method. About 7% of the final effluent wastewater samples were found positive for infectious enterovirus with the developed RF method, although only 1% of the samples were positive by presence of cytopathic effect. This method avoids the use of two complete cycles of cell culture to detect infectious virus, while confirming infectivity with a molecular method. Shipping may be from multiple locations in the US or from the UK, depending on stock availability.
- Softcover
Seller: PBShop.store US, Wood Dale, IL, U.S.A.PBShop.store US
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US$ 167.77
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PAP. Condition: New. New Book. Shipped from UK. Established seller since 2000.
- Softcover
Seller: PBShop.store UK, Fairford, GLOS, United KingdomPBShop.store UK
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PAP. Condition: New. New Book. Shipped from UK. Established seller since 2000.
- Softcover
Seller: GreatBookPricesUK, Woodford Green, United KingdomGreatBookPricesUK
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- Softcover
Seller: GreatBookPrices, Columbia, MD, U.S.A.GreatBookPrices
Contact seller5-star sellerCondition: Used - As new
US$ 185.28
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Condition: As New. Unread book in perfect condition.
- Softcover
Seller: Majestic Books, Hounslow, United KingdomMajestic Books
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US$ 182.52
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Condition: New. pp. 80 Illus.
- Softcover
Seller: Books Puddle, New York, NY, U.S.A.Books Puddle
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US$ 193.12
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Condition: New. pp. 80.
- Softcover
Seller: Ria Christie Collections, Uxbridge, United KingdomRia Christie Collections
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Condition: New. In.
- Softcover
Seller: Speedyhen, Hertfordshire, United KingdomSpeedyhen
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Condition: NEW.
- Softcover
Seller: GreatBookPricesUK, Woodford Green, United KingdomGreatBookPricesUK
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US$ 193.39
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Condition: As New. Unread book in perfect condition.
- Softcover
Seller: Rarewaves USA, HEBRON, KY, U.S.A.Rarewaves USA
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US$ 209.78
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Paperback. Condition: New. Enteroviruses, hepatitis A virus (HAV) and other enteric viruses can survive wastewater treatment processes, even after chlorination, and are found in the final effluents. These viruses can be detected by cell culture techniques with observations for cytopathic effect (CPE). Recently molecular detecti…on of viral nucleic acids has been used. Most viruses found in wastewater are RNA viruses and RT-PCR is a rapid and sensitive method to detect these single-strand RNA enteric viruses. This methodology does not distinguish between infectious and non-infectious viruses. Viruses inactivated in the treatment process can be detected but do not pose a public health threat. Methods are needed to quickly distinguish the infectious viruses from inactivated viruses, both of which may be present in effluents. In this project we investigated the use of a method that combines cell culture and molecular detection. If a sample contains viruses that replicate in cell culture even without CPE, the proof of replication can be demonstrated by the detection of a replicative form (RF) in cell culture that is only present during replication of infectious RNA viruses.
- Softcover
Seller: Rarewaves.com USA, London, LONDO, United KingdomRarewaves.com USA
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US$ 209.85
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Paperback. Condition: New. Enteroviruses, hepatitis A virus (HAV) and other enteric viruses can survive wastewater treatment processes, even after chlorination, and are found in the final effluents. These viruses can be detected by cell culture techniques with observations for cytopathic effect (CPE). Recently molecular detecti…on of viral nucleic acids has been used. Most viruses found in wastewater are RNA viruses and RT-PCR is a rapid and sensitive method to detect these single-strand RNA enteric viruses. This methodology does not distinguish between infectious and non-infectious viruses. Viruses inactivated in the treatment process can be detected but do not pose a public health threat. Methods are needed to quickly distinguish the infectious viruses from inactivated viruses, both of which may be present in effluents. In this project we investigated the use of a method that combines cell culture and molecular detection. If a sample contains viruses that replicate in cell culture even without CPE, the proof of replication can be demonstrated by the detection of a replicative form (RF) in cell culture that is only present during replication of infectious RNA viruses.
- Softcover
Seller: THE SAINT BOOKSTORE, Southport, United KingdomTHE SAINT BOOKSTORE
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US$ 200.49
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Paperback / softback. Condition: New. New copy - Usually dispatched within 3 working days.
- Softcover
Seller: Revaluation Books, Exeter, United KingdomRevaluation Books
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US$ 205.54
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Paperback. Condition: Brand New. illustrated edition. 78 pages. 10.75x8.25x0.25 inches. In Stock.
- Softcover
Seller: Kennys Bookshop and Art Galleries Ltd., Galway, GY, IrelandKennys Bookshop and Art Galleries Ltd.
Contact seller5-star sellerCondition: New
US$ 212.89
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Condition: New. 2004. paperback. . . . . .
- Softcover
Seller: Biblios, frankfurt am main, HESSE, GermanyBiblios
Contact seller4-star sellerCondition: New
US$ 218.84
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Condition: New. pp. 80.
- Softcover
Seller: moluna, Greven, Germanymoluna
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US$ 186.17
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Condition: New. KlappentextEnteroviruses, hepatitis A virus (HAV) and other enteric viruses can survive wastewater treatment processes, even after chlorination, and are found in the final effluents. These viruses can be detected by cell culture techniq.
- Softcover
Seller: Kennys Bookstore, Olney, MD, U.S.A.Kennys Bookstore
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US$ 250.31
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Condition: New. 2004. paperback. . . . . . Books ship from the US and Ireland.
- Softcover
Seller: Rarewaves USA United, HEBRON, KY, U.S.A.Rarewaves USA United
Contact seller5-star sellerCondition: New
US$ 219.34
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Paperback. Condition: New. Enteroviruses, hepatitis A virus (HAV) and other enteric viruses can survive wastewater treatment processes, even after chlorination, and are found in the final effluents. These viruses can be detected by cell culture techniques with observations for cytopathic effect (CPE). Recently molecular detecti…on of viral nucleic acids has been used. Most viruses found in wastewater are RNA viruses and RT-PCR is a rapid and sensitive method to detect these single-strand RNA enteric viruses. This methodology does not distinguish between infectious and non-infectious viruses. Viruses inactivated in the treatment process can be detected but do not pose a public health threat. Methods are needed to quickly distinguish the infectious viruses from inactivated viruses, both of which may be present in effluents. In this project we investigated the use of a method that combines cell culture and molecular detection. If a sample contains viruses that replicate in cell culture even without CPE, the proof of replication can be demonstrated by the detection of a replicative form (RF) in cell culture that is only present during replication of infectious RNA viruses.
- Softcover
Seller: preigu, Osnabrück, Germanypreigu
Contact seller5-star sellerCondition: New
US$ 205.81
US$ 81.30 shippingShips from Germany to U.S.A.Quantity: 1 available
Taschenbuch. Condition: Neu. Development of Molecular Methods for Detection of Infectious Viruses in Treated Wastewater | T. Cromeans | Taschenbuch | Werf Research Report | Kartoniert / Broschiert | Englisch | 2005 | Werf | EAN 9781843397229 | Verantwortliche Person für die EU: preigu GmbH & Co. KG, Lengericher Landstr. 19, 4907…8 Osnabrück, mail[at]preigu[dot]de | Anbieter: preigu.
- Softcover
Seller: AHA-BUCH GmbH, Einbeck, GermanyAHA-BUCH GmbH
Contact seller5-star sellerCondition: New
US$ 219.41
US$ 70.98 shippingShips from Germany to U.S.A.Quantity: 2 available
Taschenbuch. Condition: Neu. Neuware - Enteroviruses, hepatitis A virus (HAV) and other enteric viruses can survive wastewater treatment processes, even after chlorination, and are found in the final effluents. These viruses can be detected by cell culture techniques with observations for cytopathic effect (CPE). Recently molecu…lar detection of viral nucleic acids has been used. Most viruses found in wastewater are RNA viruses and RT-PCR is a rapid and sensitive method to detect these single-strand RNA enteric viruses. This methodology does not distinguish between infectious and non-infectious viruses. Viruses inactivated in the treatment process can be detected but do not pose a public health threat. Methods are needed to quickly distinguish the infectious viruses from inactivated viruses, both of which may be present in effluents. In this project we investigated the use of a method that combines cell culture and molecular detection. If a sample contains viruses that replicate in cell culture even without CPE, the proof of replication can be demonstrated by the detection of a replicative form (RF) in cell culture that is only present during replication of infectious RNA viruses. A negative sense strand of RNA is generated from the viral positive strand virus, and these two are found primarily bound in a replicative form. This RNA was specifically detected by RT-PCR, including the positive and negative strands. This assay was developed and explored for the detection of low levels of CVB3 and HAV laboratory strains first and then for low levels of wild-type enteroviruses isolated from sewage samples. As few as four infectious units of wild-type enteroviruses, contained in 2.0 ml of water concentrate prepared from 600 ml of treated wastewater effluent, could be detected within two days. Treated wastewater effluents were collected weekly over an 18 months period, and viruses were concentrated with a developed method. About 7% of the final effluent wastewater samples were found positive for infectious enterovirus with the developed RF method, although only 1% of the samples were positive by presence of cytopathic effect. This method avoids the use of two complete cycles of cell culture to detect infectious virus, while confirming infectivity with a molecular method.
- Softcover
Seller: Rarewaves.com UK, London, United KingdomRarewaves.com UK
Contact seller5-star sellerCondition: New
US$ 211.30
US$ 87.86 shippingShips from United Kingdom to U.S.A.Quantity: 5 available
Paperback. Condition: New. Enteroviruses, hepatitis A virus (HAV) and other enteric viruses can survive wastewater treatment processes, even after chlorination, and are found in the final effluents. These viruses can be detected by cell culture techniques with observations for cytopathic effect (CPE). Recently molecular detecti…on of viral nucleic acids has been used. Most viruses found in wastewater are RNA viruses and RT-PCR is a rapid and sensitive method to detect these single-strand RNA enteric viruses. This methodology does not distinguish between infectious and non-infectious viruses. Viruses inactivated in the treatment process can be detected but do not pose a public health threat. Methods are needed to quickly distinguish the infectious viruses from inactivated viruses, both of which may be present in effluents. In this project we investigated the use of a method that combines cell culture and molecular detection. If a sample contains viruses that replicate in cell culture even without CPE, the proof of replication can be demonstrated by the detection of a replicative form (RF) in cell culture that is only present during replication of infectious RNA viruses.
- Softcover
Seller: AussieBookSeller, Truganina, VIC, AustraliaAussieBookSeller
Contact seller5-star sellerCondition: New
US$ 285.35
US$ 37.00 shippingShips from Australia to U.S.A.Quantity: 1 available
Paperback. Condition: new. Paperback. Enteroviruses, hepatitis A virus (HAV) and other enteric viruses can survive wastewater treatment processes, even after chlorination, and are found in the final effluents. These viruses can be detected by cell culture techniques with observations for cytopathic effect (CPE). Recently molecul…ar detection of viral nucleic acids has been used. Most viruses found in wastewater are RNA viruses and RT-PCR is a rapid and sensitive method to detect these single-strand RNA enteric viruses. This methodology does not distinguish between infectious and non-infectious viruses. Viruses inactivated in the treatment process can be detected but do not pose a public health threat. Methods are needed to quickly distinguish the infectious viruses from inactivated viruses, both of which may be present in effluents. In this project we investigated the use of a method that combines cell culture and molecular detection. If a sample contains viruses that replicate in cell culture even without CPE, the proof of replication can be demonstrated by the detection of a replicative form (RF) in cell culture that is only present during replication of infectious RNA viruses. Enteroviruses, hepatitis A virus (HAV) and other enteric viruses can survive wastewater treatment processes, even after chlorination, and are found in the final effluents. These viruses can be detected by cell culture techniques with observations for cytopathic effect (CPE). Recently molecular detection of viral nucleic acids has been used. Most viruses found in wastewater are RNA viruses and RT-PCR is a rapid and sensitive method to detect these single-strand RNA enteric viruses. This methodology does not distinguish between infectious and non-infectious viruses. Viruses inactivated in the treatment process can be detected but do not pose a public health threat. Methods are needed to quickly distinguish the infectious viruses from inactivated viruses, both of which may be present in effluents. In this project we investigated the use of a method that combines cell culture and molecular detection. If a sample contains viruses that replicate in cell culture even without CPE, the proof of replication can be demonstrated by the detection of a replicative form (RF) in cell culture that is only present during replication of infectious RNA viruses. A negative sense strand of RNA is generated from the viral positive strand virus, and these two are found primarily bound in a replicative form. This RNA was specifically detected by RT-PCR, including the positive and negative strands. This assay was developed and explored for the detection of low levels of CVB3 and HAV laboratory strains first and then for low levels of wild-type enteroviruses isolated from sewage samples. As few as four infectious units of wild-type enteroviruses, contained in 2.0 ml of water concentrate prepared from 600 ml of treated wastewater effluent, could be detected within two days. Treated wastewater effluents were collected weekly over an 18 months period, and viruses were concentrated with a developed method. About 7% of the final effluent wastewater samples were found positive for infectious enterovirus with the developed RF method, although only 1% of the samples were positive by presence of cytopathic effect. This method avoids the use of two complete cycles of cell culture to detect infectious virus, while confirming infectivity with a molecular method. Shipping may be from our Sydney, NSW warehouse or from our UK or US warehouse, depending on stock availability.
