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Hardcover. Condition: gut. 2010 | 3rd ed. 2011. Fully revised and updated, Flow Cytometry Protocols highlights the expanding contribution of flow cytometry to basic biological research and diagnostic medicine. The text presents cutting-edge topics, such as polychromatic, quantitative, and high throughput flow cytometry, and more. With rapid improvements in instrumentation, lasers, fluorophores, and data analysis software, flow cytometry is riding the crest of unprecedented, innovative advances. This thoroughly revised and up-to-date third edition of Flow Cytometry Protocols highlights the expanding contribution of flow cytometry to basic biological research and diagnostic medicine. Written by leading experts in the field, the book presents cutting-edge topics such as polychromatic, quantitative, and high throughput flow cytometry, novel multiparametric data analysis which breaks the dimensionality barrier, standard practice and safety measures for aerosol-generating cell sorting, conventional and imaging flow cytometry as well as minimalist imaging cytometry. As a volume in the highly successful Methods in Molecular Biology series, chapters contain brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and extensive notes on troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, Flow Cytometry Protocols, Third Edition presents established as well as new flow cytometric methodologies in order to introduce beginning users to basic applications while opening new avenues of innovation for seasoned users. Content: 1. Flow Cytometry: An Introduction Alice L. Givan 2. Breaking the Dimensionality Barrier C. Bruce Bagwell 3. Quantitative Fluorescence Measurements with Multicolor Flow Cytometry Lili Wang, Adolfas K. Gaigalas, and Ming Yan 4. Quantum Dots for Quantitative Flow Cytometry Yang Wu, Larry A. Sklar, and Tione Buranda 5. Bead-Based Multiplexed Analysis of Analytes by Flow Cytometry Henri C. van der Heyde and Irene Gramaglia 6. Flow-Based Combinatorial Antibody Profiling: An Integrated Approach to Cell Characterization Shane Bruckner, Ling Wang, Ruiling Yuan, Perry Haaland, and Amitabh Gaur 7. Tracking Immune Cell Proliferation and Cytotoxic Potential Using Flow Cytometry Joseph D. Tario, Jr., Katharine A. Muirhead, Dalin Pan, Mark E. Munson, and Paul K. Wallace 8. Multiparameter Intracellular Cytokine Staining Patricia Lovelace and Holden T. Maecker 9. Phospho Flow Cytometry Methods for Analysis of Kinase Signaling in Cell Lines and Primary Human Blood Samples Peter O. Krutzik, Angelica Trejo, Kenneth R. Schulz, and Garry P. Nolan 10. Multiparametric Analysis of Apoptosis by Flow Cytometry William G. Telford, Akira Komoriya, Beverly Z. Packard, and C. Bruce Bagwell 11. Multiparameter Cell Cycle Analysis James W. Jacobberger, R. Michael Sramkoski, and Tammy Stefan 12. Rare Event Detection and Analysis in Flow Cytometry: Bone Marrow Mesenchymal Stem Cells, Breast Cancer Stem/Progenitor Cells in Malignant Effusions, and Pericytes in Disaggregated Adipose Tissue Ludovic Zimmerlin, Vera S. Donnenberg, and Albert D. Donnenberg 13. Flow Cytometric-Based Identification of Immature Myeloerythroid Development Cornelis J.H. Pronk and David Bryder 14. Flow Cytometry Immunophenotyping of Hematolymphoid Neoplasia Katherine R. Calvo, Catharine S. McCoy, and Maryalice Stetler-Stevenson 15. Flow Cytometry Assays in Primary Immunodeficiency Diseases Maurice R.G. O'Gorman, Joshua Zollett, and Nicolas Bensen 16. Flow Cytometric Analysis of Microparticles Henri C. van der Heyde, Irene Gramaglia, Valery Combes, Thaddeus C. George, and Georges E. Grau 17. Noncytotoxic DsRed Derivatives for Whole-Cell Labeling Rita L. Strack, Robert J. Keenan, and Benjamin S. Glick 18. Flow Cytometric FRET Analysis of Protein Interaction Gyoergy Vereb, Peter Nagy, and Janos Szoellosi 19. Fluorescent Protein-Assisted Purification for Gene Expression Profiling M. Raza Zaidi, Chi-Ping Day, and Glenn Merlino 20. Multiparametric Analysis, Sorting, and Transcriptional Profiling of Plant Protoplasts and Nuclei According to Cell Type David W. Galbraith, Jaroslav Janda, and Georgina M. Lambert 21. Lentiviral Fluorescent Protein Expression Vectors for Biotinylation Proteomics Irene Riz, Teresa S. Hawley, and Robert G. Hawley 22. Standard Practice for Cell Sorting in a BSL-3 Facility Stephen P. Perfetto, David R. Ambrozak, Richard Nguyen, Mario Roederer, Richard A. Koup, and Kevin L. Holmes 23. The Cytometric Future: It Ain't Necessarily Flow! Howard M. Shapiro Reihe/Serie Methods in Molecular Biology ; 699 Zusatzinfo 26 Tables, black and white; XII, 486 p. Verlagsort Totowa, NJ Sprache englisch Maße 178 x 254 mm Studium 1. Studienabschnitt Vorklinik Histologie Embryologie Studium 2. Studienabschnitt Klinik Anamnese Körperliche Untersuchung Naturwissenschaften Biologie Genetik Molekularbiologie Technik Umwelttechnik Biotechnologie ISBN-10 1-61737-949-2 / 1617379492 ISBN-13 978-1-61737-949-9 / 9781617379499 Flow Cytometry Protocols Methods in Molecular Biology Band 699 [Hardcover] Hawley, Teresa S. Hawley, Robert In englischer Sprache. 485 pages. 178 x 254 mm.
Seller: Biblios, Frankfurt am main, HESSE, Germany
Condition: New. pp. 460.
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Published by Elsevier Science Publishing Co Inc, 2020
ISBN 10: 0128160551 ISBN 13: 9780128160558
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Taschenbuch. Condition: Neu. Urban Systems Design | Creating Sustainable Smart Cities in the Internet of Things Era | Yoshiki Yamagata (u. a.) | Taschenbuch | Einband - fest (Hardcover) | Englisch | 2020 | Elsevier | EAN 9780128160558 | Verantwortliche Person für die EU: preigu GmbH & Co. KG, Lengericher Landstr. 19, 49078 Osnabrück, mail[at]preigu[dot]de | Anbieter: preigu.
Seller: moluna, Greven, Germany
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Add to basketCondition: New. Provides operational case studies and best practices from cities throughout Europe, North America, Latin America, Asia, Australia, and Africa, providing instructive examples of the social, environmental, and economic aspects of smartification?
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Published by Elsevier Science & Technology, Elsevier, 2020
ISBN 10: 0128160551 ISBN 13: 9780128160558
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Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Urban Systems Design: Creating Sustainable Smart Cities in the Internet of Things Era shows how to design, model and monitor smart communities using a distinctive IoT-based urban systems approach. Focusing on the essential dimensions that constitute smart communities energy, transport, urban form, and human comfort, this helpful guide explores how IoT-based sharing platforms can achieve greater community health and well-being based on relationship building, trust, and resilience. Uncovering the achievements of the most recent research on the potential of IoT and big data, this book shows how to identify, structure, measure and monitor multi-dimensional urban sustainability standards and progress. This thorough book demonstrates how to select a project, which technologies are most cost-effective, and their cost-benefit considerations. The book also illustrates the financial, institutional, policy and technological needs for the successful transition to smart cities, and concludes by discussing both the conventional and innovative regulatory instruments needed for a fast and smooth transition to smart, sustainable communities. Englisch.
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Published by Elsevier Science Ltd, 2020
ISBN 10: 0128160551 ISBN 13: 9780128160558
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Add to basketPaperback. Condition: Brand New. 432 pages. 8.75x6.00x1.25 inches. In Stock. This item is printed on demand.
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Published by Elsevier Science Publishing Co Inc, 2020
ISBN 10: 0128160551 ISBN 13: 9780128160558
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ISBN 10: 0128160551 ISBN 13: 9780128160558
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Taschenbuch. Condition: Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - Urban Systems Design: Creating Sustainable Smart Cities in the Internet of Things Era shows how to design, model and monitor smart communities using a distinctive IoT-based urban systems approach. Focusing on the essential dimensions that constitute smart communities energy, transport, urban form, and human comfort, this helpful guide explores how IoT-based sharing platforms can achieve greater community health and well-being based on relationship building, trust, and resilience. Uncovering the achievements of the most recent research on the potential of IoT and big data, this book shows how to identify, structure, measure and monitor multi-dimensional urban sustainability standards and progress. This thorough book demonstrates how to select a project, which technologies are most cost-effective, and their cost-benefit considerations. The book also illustrates the financial, institutional, policy and technological needs for the successful transition to smart cities, and concludes by discussing both the conventional and innovative regulatory instruments needed for a fast and smooth transition to smart, sustainable communities.