springer new york jun 2018 (164 results)

Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume presents techniques and recently developments in extracellular recording. The chapters in this book explore topics such as mouse, rat and zebrafish models; extracellular recordings on human cognition; single-unit recordings and multi-electrode arrays; and recordings in perinatal and early developing mice. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.Cutting-edge and authoritative, Extracellular Recording Approaches is a valuable resource that provides readers with up-to-date applications and methods to study brain regions involved in sensory, motor, and cognitive functions. 312 pp. Englisch.

Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Current worldwide trends are not sustainable. The Club of Rome's warnings published in the book Limits to Growth are still valid. Remedies that are acceptable for the great majority tend to make things worse. We seem to be in a philosophical crisis. Pope Francis says it clearly: our common home is in deadly danger. Analyzing the philosophical crisis, the book comes to the conclusion that the world may need a 'new enlightenment'; one that is not based solely on doctrine, but instead addresses a balance between humans and nature, as well as a balance between markets and the state, and the short versus long term. To do this we need to leave behind working in 'silos' in favor of a more systemic approach that will require us to rethink the organization of science and education.However, we have to act now; the world cannot wait until 7.6 billion people have struggled to reach a new enlightenment.This book is full of optimistic case studies and policy proposals that will lead us back to a trajectory of sustainability. But it is also necessary to address the taboo topic of population increase. Countries with a stable population fare immensely better than those with continued increase. Finally, we are presenting an optimistic book from the Club of Rome. 236 pp. Englisch.

Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume provides properties, biological function, methods,and approaches for manipulating nAChRs in different organisms. NicotinicAcetylcholine Receptor Technologies guides readers through moleculartechniques and behavioral tests used to investigate nicotinic drugs,chronoamperometry, emerging technologies and methods for the analysis ofnAChRs, single nucleotide polymorphism (SNP), fluorescence techniques, spectralconfocal, allosteric modulators of a7-nAChRs, and a comprehensive evolutionalrelation for most nAChR subunits, in both vertebrate and invertebrate species.Written for the popular Neuromethodsseries, chapters include thekind of detail and key implementation advice that ensures successful results inthe laboratory.Authoritative and practical,Nicotinic AcetylcholineReceptor Technologies aimsto ensure successful results in thefurther study of this vital field. 272 pp. Englisch.

Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -The revised second edition of this textbook provides the reader with a solid foundation in probability theory and statistics as applied to the physical sciences, engineering and related fields. It covers a broad range of numerical and analytical methods that are essential for the correct analysis of scientific data, including probability theory, distribution functions of statistics, fits to two-dimensional data and parameter estimation, Monte Carlo methods and Markov chains.Features new to this edition include:- a discussion of statistical techniques employed in business science, such as multiple regression analysis of multivariate datasets.- a new chapter on the various measures of the mean including logarithmic averages.- new chapters on systematic errors and intrinsic scatter, and on the fitting of data with bivariate errors.- a new case study and additional worked examples.- mathematical derivations and theoretical background material have been appropriately marked, to improve the readability of the text.- end-of-chapter summary boxes, for easy reference.As in the first edition, the main pedagogical method is a theory-then-application approach, where emphasis is placed first on a sound understanding of the underlying theory of a topic, which becomes the basis for an efficient and practical application of the material. The level is appropriate for undergraduates and beginning graduate students, and as a reference for the experienced researcher. Basic calculus is used in some of the derivations, and no previous background in probability and statistics is required. The book includes many numerical tables of data, as well as exercises and examples to aid the readers' understanding of the topic. 336 pp. Englisch.

Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume is the result of an explosion ofmolecular-based research on Cilia, which began with the discovery of theuniversality of intraflagellar transport (IFT) and ciliary genomics/proteomics.The chapters in this book cover topics such as: high resolution imaging andfunctional characterization of sensory and primary cilia in mammalian cells andzebrafish, methods to study ciliary-mediated chemoresponse in Paramecium,and methods to study centrosomes and cilia in C. elegans and Drosophila.Written in the highly successful Methods in Molecular Biology series format,chapters include introduction to their respective topics, lists of thenecessary materials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls.Practical and cutting-edge, Cilia:Methods and Protocols is broad and covers motile, sensory, and primarycells. It is a valuable resource to anyone interested in entering the field ofciliary biology using model organisms, including flagellate algae, ciliates,planaria, nematodes, insects, zebrafish, and mammalian cells. 276 pp. Englisch.

Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This fully updated and revised second edition address specifically some of the significant advances made in our understanding of the pathophysiology of penile cancer including discussion of the suspected role of the human papilloma virus in penile carcinogenesis. Similarly, the molecular pathways implicated in precancerous and penile cancer will be discussed as these are the present targets of novel therapeutic approaches to this disease. The text details the recent national and international diagnostic and treatment guidelines that have been developed by both the National Comprehensive Cancer Network and the European Urological Association establishing evidence based standards upon which the care of penile cancer should be based. The text also covers significant advances made in the diagnostic and therapeutic approach to both primary tumors and inguinal nodal metastases resulting from penile cancer such as penile sparing surgery and minimally invasive inguinal lymph node surgery. Thetext will similarly highlights the improvement in cancer specific outcome in the management of loco-regionally advanced (N2/3) penile cancer managed by upfront systemic chemotherapy followed by consolidative surgery in appropriately selected patients based on recent prospective phase II data. It also covers the role of radiotherapy in the management of penile cancer for both primary tumors and/or at sites of regional or distant metastases. Finally, some of the novel systemic approaches being devised in the management of advanced penile cancer along with some upcoming international trials soon to be opened which likely will re-define the present therapeutic approaches to penile cancer are discussed.The Second Edition of Penile Cancer: Diagnosis and Treatment will constitute a comprehensive review of the above mentioned subjects in addition to a few select topics of high clinical relevance, with the readership predominantly consisting of urologists, medical and surgical oncologists, and other healthcare professionals. 164 pp. Englisch.

Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume presents relevantbackground information to understanding the molecular basis governingunconventional protein secretion (UPS), and in particular explores the latesttechniques and protocols that have been successfully applied for the study ofthis topic. Detailed chapters include an overview of conventional andunconventional secretory pathways along with multidisciplinary approaches andmethods used for UPS analysis in different organisms. Written in the highly successful Methods inMolecular Biology series format, chapters include introductions to theirrespective topics, lists of the necessary materials and reagents, step-by-step,readily reproducible laboratory protocols, and tips on troubleshooting andavoiding known pitfalls.Authoritative and cutting-edge, UnconventionalProtein Secretion: Methods and Protocols will be usefulfor all interested in the secretory pathway field as well as applications incell biology, cell development, biomedical research, and healthcare. 288 pp. Englisch.

Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This book is open access under a CC BY 4.0 license.This book provides a practical and self-contained overview of the Gene Ontology (GO), the leading project to organize biological knowledge on genes and their products across genomic resources. Written for biologists and bioinformaticians, it covers the state-of-the-art of how GO annotations are made, how they are evaluated, and what sort of analyses can and cannot be done with the GO. In the spirit of the Methods in Molecular Biology book series, there is an emphasis throughout the chapters on providing practical guidance and troubleshooting advice.Authoritative and accessible, The Gene Ontology Handbook serves non-experts as well as seasoned GO users as a thorough guide to this powerful knowledge system. 320 pp. Englisch.

Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume presents classical approaches to in vivo neuropharmacology and neurophysiology, such as c-fos, electrochemistry, microdialysis microstimulation, and push-up superfusion. It also explores exciting new methods for behavioral analysis, and techniques based on optogenetics and non-invasive magnetic resonance imaging. The chapters of this book cover topics such as principles of stereotaxy, pharmaco-based fMRI and neurophysiology in humans and non-human primates, electrical nerve stimulation and central microstimulation, involvement of neurotransmitters in mnemonic processes, the impact of cannabinoids on motor activity, as well as the involvement of nitric oxide in neurotoxicity produced by psychostimulant drugs. Each chapter also discusses difficulties, tips, tricks, and precautions to take. Neuromethods series style chapters include the kind of detail and key advice from the specialists needed to get successful results in your own laboratory.Cutting-edge andpractical, In Vivo Neuropharmacology and Neurophysiology is a valuable resource for experienced and less experienced investigators of brain function and brain disorders. 444 pp. Englisch.

Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This textbook consists primarily of notes by Iain Finnie who taught a popular course on fracture mechanics at the University of California at Berkeley. It presents a comprehensive and detailed exposition of fracture, the fundamentals of fracture mechanics and procedures for the safe design of engineering components made from metal alloys, brittle materials like glasses and ceramics, and composites. Interesting and practical problems are listed at the end of most chapters to give the student practice in applying the theory. A solutions manual is provided to the instructor. The text presents a unified perspective of fracture with a strong fundamental foundation and practical applications. In addition to its role as a text, this reference would be invaluable for the practicing engineer who is involved in the design and evaluation of components that are fracture critical.This book also:Presents details of derivations of the basic equations of fracture mechanics and the historical context of the development of fracture theory and methodologyTreats linear and nonlinear fracture mechanics methodologies beginning with a review of the basic equations of solid mechanics followed by solutions useful in fracture predictionIllustrates the basis of linear elastic fracture mechanics (LEFM), practical applications of LEFM in the design of fracture-tolerant structural componentsOffers interesting, practical, classroom proven problems at the end of most chaptersIncludes instructor's solutions manual 268 pp. Englisch.

Buch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume explores current viewpoints and knowledge gaps in the field of traumatic brain injury (TBI). The chapters in this book cover topics ranging from development of in vitro and animal TBI models, to diagnostic imaging and disease monitoring in patients. Designing pre-clinical and clinical trials is also discussed. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.Practical and thorough, Pre-Clinical and Clinical Methods in Brain Trauma Research is a valuable resource for both scientists and clinical researchers interested in learning about important techniques and their applications in the field of TBI. 396 pp. Englisch.

Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This stimulating volume examines the many faces of Australia's ageing population, the social and health issues they contend with, and the steps being taken-and many that should be taken-to help ensure a more positive and productive later life. Individual and societal ageing are conceptualized as developmental in nature, socially diverse, and marked by daily life challenges stemming from the country's economic structures, attitudes, geography, political landscape, and infrastructure. Wide-ranging coverage (e.g., health, inequalities, employment, transportation) assesses options available to older people, and the role of families, employers, service providers, government agencies, and others in promoting or expanding those choices. The book's double emphasis on challenges in older people's lives and opportunities for enhancing their quality of life is on clear display as case studies examine policy issues-and propose solutions-in a societal and individual context.Included in the coverage: Australian developments in ageing: issues and history. Cultural diversity, health, and ageing. Indigenous Australians and ageing: responding to diversity in policy and practice. Enhancing the health and employment participation of older workers. Housing and the environments of ageing. Health services and care for older people.The rich examples inAgeing in Australiacontain a depth of understanding and evidence for sociologists, gerontologists and psychologists studying ageing, health care professionals providing care to older people, and policy analysts assessing areas for improvement. 304 pp. Englisch.

Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume examines the structure and dynamics of the bacterial flagellum using bacterial genetics, molecular biology, biochemistry, structural biology, biophysics, cell biology, and molecular dynamics simulation. The chapters are divided into 4 parts: Part I describes flagellar type III protein exports, assembly, and gene regulation in S. enterica; Part II explains how to isolate the flagella from the bacterial cell bodies, and further explains how to conduct high-resolution structural and functional analyses of the flagellar motor; Part III talks about how to measure flagellar motor rotation over a wide range of external load, how to measure ion motive force across the cytoplasmic membrane, and how to measure dynamic properties of the flagellar motor proteins by fluorescence microscopy with single molecule precision; and Part IV explores the structure and function of Spirochetal, Vibrio, Shewanella, and Magnetococcus flagellar motors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, The Bacterial Flagellum: Methods and Protocols aims to provide valuable and vital research to aid in the investigation of the bacterial flagellum resulting from various bacterial species. 340 pp. Englisch.

Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This guide provides a comprehensive review of the full spectrum of hypertension in chronic kidney disease (CKD). Targeted towards the busy practitioner, the focus of this volume is on various therapies and how to lower blood pressure through lifestyle changes. Specialist patient populations and hypertension and causes of hypertension are also covered in detail. Clinically-focused and authoritative, this resource offers a rationalized approach to diagnosing and treating hypertension in CKD. 360 pp. Englisch.

Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume expands and updates the coverage in the authors' popular 1992 book, Electron Microdiffraction. As the title implies, the focus of the book has changed from electron microdiffraction and convergent beam electron diffraction to all forms of advanced transmission electron microscopy. Special attention is given to electron diffraction and imaging, including high-resolution TEM and STEM imaging, and the application of these methods to crystals, their defects, and nanostructures. The authoritative text summarizes and develops most of the useful knowledge which has been gained over the years from the study of the multiple electron scattering problem, the recent development of aberration correctors and their applications to materials structure characterization, as well as the authors' extensive teaching experience in these areas. Advanced Transmission Electron Microscopy: Imaging and Diffraction in Nanoscience is ideal for use as an advanced undergraduate or graduatelevel text in support of course materials in Materials Science, Physics or Chemistry departments. 756 pp. Englisch.

Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This book will move the field of pediatric cochlear implantation forward by educating clinicians in the field as to current and emerging best practices and inspiring research in new areas of importance, including the relationship between cognitive processing and pediatric cochlear implant outcomes. The book discusses communication practices, including sign language for deaf children with cochlear implants and the role of augmentative/alternative communication for children with multiple disabilities. Focusing exclusively on cochlear implantation as it applies to the pediatric population, this book also discusses music therapy, minimizing the risk of meningitis in pediatric implant recipients, recognizing device malfunction and failure in children, perioperative anesthesia and analgesia considerations in children, and much more.Cochlear Implants in Children is aimed at clinicians, including neurotologists, pediatric otolaryngologists, audiologists and speech-language pathologists, as well as clinical scientists and educators of the deaf. The book is also appropriate for pre-and postdoctoral students, including otolaryngology residents and fellows in Neurotology and Pediatric Otolaryngology. 384 pp. Englisch.

Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This graduate-level textbook is primarily aimed at graduate students of statistics, mathematics, science, and engineering who have had an undergraduate course in statistics, an upper division course in analysis, and some acquaintance with measure theoretic probability. It provides a rigorous presentation of the core of mathematical statistics.Part I of this book constitutes a one-semester course on basic parametric mathematical statistics. Part II deals with the large sample theory of statistics - parametric and nonparametric, and its contents may be covered in one semester as well. Part III provides brief accounts of a number of topics of current interest for practitioners and other disciplines whose work involves statistical methods. 404 pp. Englisch.

Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This second edition provides updated and expanded chapters covering a broad sampling of useful and current methods in the rapidly developing and expanding field of bioinformatics. Bioinformatics, Volume I: Data, Sequence Analysis, and Evolution, Second Edition is comprised of three sections: Data and Databases, Sequence Analysis, and Phylogenetics and Evolution. The first section details bioinformatics methodologies in the generation of sequence and structural data and its organization into conceptual categories, and databases to facilitate further analyses. The Sequence Analysis section describes the fundamental methodologies for processing the sequences of biological molecules: techniques that are used in almost every pipeline of bioinformatics analysis, particularly in the preliminary stages of such pipelines. Last but not least, the phylogenetics and evolution section deals with methodologies that compare biological sequences for the purpose of understanding how they evolved. As a volume in the highly successful Methods in Molecular Biologyseries, chapters feature the kind of detail and expert implementation advice to ensure positive results.Comprehensive and practical, Bioinformatics, Volume I: Data, Sequence Analysis, and Evolution, Second Edition is an essential resource for graduate students, early career researchers, and others who are in the process of integrating new bioinformatics methods into their research. 504 pp. Englisch.

Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume reviews and summarizes some of A. I. McLeod's significant contributions to time series analysis. It also contains original contributions to the field and to related areas by participants of the festschrift held in June 2014 and friends of Dr. McLeod. Covering a diverse range of state-of-the-art topics, this volume well balances applied and theoretical research across fourteen contributions by experts in the field. It willbe of interest to researchers and practitioners in time series, econometricians, and graduate students in time series or econometrics, as well as environmental statisticians, data scientists, statisticians interested in graphical models, and researchers in quantitative risk management. 304 pp. Englisch.

Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Small molecule microarrays (SMM) were introduced in 1999 and within just a short span of time, have established themselves as a vibrant next generation platform for high-throughput screening. Small Molecule Microarrays: Methods and Protocols consolidates a significant collection of examples, which serve to guide researchers toward ways in which SMM technology may be effectively deployed for multiplexed screening, drug discovery and ligand identification. The volume is organized into three sections covering microarray fabrication, imaging modes, and discovery approaches. The chapters cover a range of small molecule library types, ranging from synthetic combinatorial libraries to peptide and carbohydrate libraries, with lessons and techniques that can be widely applied. Written in the highly successful Methods in Molecular Biology series, format, chapters include introductions of their respective topics, lists of the requisite materials and reagents, step-by-step and readily reproducible laboratory protocols as well as vital tips on troubleshooting and advice on avoiding known pitfalls.Authoritative and cutting-edge, Small Molecule Microarrays: Methods and Protocols provides meticulous insights into the successful applications of SMM, by experts in the field. Building on this collection of knowledge, the volume seeks to inspire a future generation of microarray practitioners to propel this significant technology to even greater heights 272 pp. Englisch.

Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume presents methods and techniques to studyoogenesis in a broad range of organisms, from plants to mammals. Oogenesis: Methods and Protocols guidesreaders through protocols on models ofdevelopmental biology, oogenesis in plants, worms, fruit flies,mosquitos, butterflies, starfish, zebrafish, frog, chicken and mouse. Written in the highly successful Methodsin Molecular Biology series format, chapters include introductions to theirrespective topics, lists of the necessary materials and reagents, step-by-step,readily reproducible laboratory protocols, and tips on troubleshooting andavoiding known pitfalls.Authoritative and cutting-edge, Oogenesis:Methods and Protocols aims to ensure successful results in the furtherstudy of this vital field. 288 pp. Englisch.

Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume presents a collection of tools currently used for the characterization of rust, the host plant wheat, and their interactions. This book is divided into five parts: Parts I and II discuss advanced techniques for characterizing rust pathogens in rust surveillance, genotyping, and molecular pathogenicity; Part III describes protocols for genetic analysis of rust resistance; Part IV covers methods on rust resistance gene cloning; and Part V talks about the isolation and screening of bacterial endophytes as biocontrol agents for rust disease management. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and authoritative, Wheat Rust Disease: Methods and Protocols is a valuable resource for both established and novel wheat rust researchers and also the plant science and microbial research community. 308 pp. Englisch.

Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume aims to provide a range of methods and protocols for studying tumor angiogenesis in vitro and in vivo to reflect advances in the field. The chapters in this book cover topics such as: morphological aspects of tumor angiogenesis, aortic ring assay and its use for the study of tumor angiogenesis, ex vivo tissue culture model for anti-angiogenic drug testing, transgenic zebrafish, orthotopic models of ovarian cancer, and uncovering metabolic effects of anti-angiogenic therapy in tumors by induced metabolic bioluminescence imaging. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting edge and thorough, Tumor Angiogenesis Assays: Methods and Protocols is a valuable resource for anyone interested in tumor angiogenesis assay research. 208 pp. Englisch.

Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume covers arange of methods used in plant cytogenetics, beginning with basic analysis ofchromosomes and visualizing gene locations, to manipulating and dissectingchromosomes, and then focusing on less understood features of chromosomes suchas recombination initiation sites and epigenomic marks. The methods describedin Plant Cytogenetics: Methods and Protocols build on each other andprovide, those new to the field, with a comprehensive platform to support theirresearch endeavours, while also introducing advanced techniques to experiencedresearchers. Written in the highly successful Methods in Molecular Biology seriesformat, chapters include introductions to their respective topics, lists of thenecessary materials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls.Cuttingedge and thorough, Plant Cytogenetics: Methods and Protocols, is avaluable resource for anyone who is interested in the diverse and wonderfullycomplex field of cytogenetics. 212 pp. Englisch.

Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume highlights the molecular and cellular methods used in studyingChronic Myeloid Leukimia (CML)pathogenesis and stem cell biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Chronic Myeloid Leukemia: Methods and Protocols aims to ensure successful results in the further study of this vital field. 272 pp. Englisch.

Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Thisvolume summarizes current techniques; ranging from protocols for virus growth,isolation, quantification and generation of recombinant RSV virus to proceduresfor the efficient characterization of the host immune response to RSVinfection. These techniques are used in numerous laboratories around the worldand are the building blocks that support the majority of RSV virus research. Written in the highly successful Methods in MolecularBiology series format, chapters include introductions to their respectivetopics, lists of the necessary materials and reagents, step-by-step, readilyreproducible laboratory protocols, and tips on troubleshooting and avoidingknown pitfalls.Authoritative and cutting-edge, Humana Respiratory Syncytial Virus:Methods and Protocols aims to ensure successful resultsin the further study of this vital field. 260 pp. Englisch.

Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume explores a broad range of different genotyping techniques.Genotyping: Methods and Protocols consists of chapters that cover numerous topics such as: an overview of multiplexed microsatellite analysis; High Resolution Melt analysis and TaqMan-based assays; in situ analysis of variants in single RNA molecules; the MassARRAY system and Molecular Inversion Probes; Pulsed Field Gel Electrophoresis, Paralogue Ratio Test, and Multiplex Ligation-Dependent Probe Amplification; long-range PCR combined with PacBio sequencing; Targeted Locus Amplification; Multilocus Sequence Typing and rapid SNP detection with pyrosequencing; and genotyping-by-sequencing for plant analysis. Finally, the volume concludes with a summary of pertinent points to describe genetic variation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Thorough and practical, Genotyping: Methods and Protocols is a valuable resource for anyone interested in learning more about the diverse field of genotyping. 264 pp. Englisch.

Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume provides a comprehensive overview of the experimental and computational methodologies used to study the function of long non-coding RNA (ncRNAs) expressed from enhancers. Chapter detail both wet-lab and dry-lab techniques and annotating long ncRNAs and exploring transcription by assessing where transcription starts and generally how it occurs.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Enhancer RNAs: Methods and Protocols aims to ensure successful results in this rapidly developing field. 268 pp. Englisch.

Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume provides an up-to-date compilationof current methodological approaches utilized for the exploration of nucleolarstructure and function. Chapters covera diversity of protocols that include imaging of the nucleolus, analysis ofribosomal RNA transcription and processing, and genomics and proteomics of thenucleolus. Written in the highly successful Methods in Molecular Biology seriesformat, chapters include introductions to their respective topics, lists of thenecessary materials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, The Nucleolus: Methods and Protocols provides scientists with a reliable practicalhandbook to facilitate the investigation of this nuclear compartment at theadvanced level. 292 pp. Englisch.

Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Now poised to evaluate the potential in modulating Wnt signaling for therapeutic agendas in cancer, wound healing, and degenerative disease, this book collects protocols from an exciting area of study. The methodologies include using Wnt modulating chemicals in engineering tissues from induced pluripotent and embryonic stem cells, monitoring Wnt transcriptional responses in diverse tissues such as bone and skin, and using specific biochemical markers of Wnt signaling to either screen molecular libraries or evaluate novel reagents. These protocols also leverage unique experimental strengths from five different model organisms. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Practical and authoritative, Wnt Signaling: Methods and Protocols aims to support researchers in understanding Wnt signal transduction in any effort to improve outcomes in regenerative medicine and cancer management. 212 pp. Englisch.