Animal Genomics for Conservation and Production: A Practical Approach

Contents: Preface. 1. Laboratory practices for conducting molecular biology experiment/Minakshi Dange and K. Dushyanth. 2. Isolation of nucleic acids from different tissues/cells/Minakshi Dange and T.K. Bhattacharya. 3. Quantification and purity analysis of DNA/T.K. Bhattacharya. 4. Polymerase chain reaction/Minakshi Dange and Chandan Paswan. 5. Quantitative polymerase chain reaction (qPCR)/Minakshi Dange and T.K. Bhattacharya. 6. Agarose gel electrophoresis/K. Dushyanth and Chandan Paswan. 7. Polyacrylamide gel electrophoresis/T.K. Bhattacharya and R.N. Chatterjee. 8. Molecular biology lab establishment/Minakshi Dange. 9. Gene cloning and expression in prokaryotic system/T.K. Bhattacharya. 10. SDS poly acrylamide gel electrophoresis SDS-PAGE/S. Dhanasekaran and T.K. Bhattacharya. 11. Microsatellite typing/R.N. Chatterjee and Minakshi Dange. 12. Single stranded conformation polymorphism for genotyping/K. Dushyanth, R.N. Chatterjee and T.K. Bhattacharya. 13. Animal cell culture/T.K. Bhattacharya and S. Dhanasekaran. 14. Lymphocyte culture/Minakshi Dange. 15. Exploration of epigenetic modulation/Chandan Paswan and T.K. Bhattacharya. 16. Bio-statistics applied in animal experimentation/T.K. Bhattacharya. 17. Role of bioinformatics/T.K. Bhattacharya. 18. Semen biotechnology/M. Shanmugan. Due to constant demand of ever increasing human population, like food crops animal products are also in great demand to abolish malnutrition being faced by the people of developing countries and to enrich our health status. India is stepping towards being a developed country for which primary need is food security and safety. The advanced biotechnological tools can enhance the productivity of livestock and poultry through application of genomics in breeding programme to mitigate the constant demand. For exploring genomic profile handling of cell DNA, RNA protein is necessary. This book entails principles and approaches about several molecular tools, which are required for genomics study of animals, fishes and plants. The book covers a number of important topics right from the basics to advanced so that beginners who want to start molecular biology works in the laboratory as well as experts who want to do more advanced study in this area. …