Enzyme Engineering and Evolution: General Methods (Methods in Enzymology, Volume 643)

Good+. Hardcover, xvi + 343 pages, NOT ex-library. Interior is clean and bright with unmarked text, free of inscriptions and stamps, firmly bound. Faint external shelfwear, bumped boards edges with creases to corners. Issued without a dust jacket. -- An authoritative and comprehensive overview of the techniques and methodologies used in the engineering and evolution of enzymes. It delves into the principles and applications of enzyme engineering, highlighting advances in directed evolution, rational design, and computational methods. The book is an invaluable resource for biochemists, molecular biologists, and researchers seeking to understand and harness the power of enzymes for various scientific and industrial applications. With contributions from leading experts, it covers a range of topics from basic concepts to advanced experimental protocols. -- Contents: Preface; 1. In Vivo Site-directed Recombination (SDR): An Efficient Tool to Reveal Beneficial Epistasis; 2. A Beginner's Guide to Molecular Dynamics Simulations and the Identification of Cross-correlation Networks for Enzyme Engineering; 3. Exploration of Enzyme Diversity: High-throughput Techniques for Protein Production and Microscale Biochemical Characterization; 4. Emulsion-based Directed Evolution of Enzymes and Proteins in Yeast; 5. Remodeling Enzyme Active Sites by Stepwise Loop Insertion; 6. Consensus Finder Web Tool to Predict Stabilizing Substitutions in Proteins; 7. The Use of Consensus Sequence Information to Engineer Stability and Activity in Proteins; 8. Structure-guided Rational Design of the Substrate Specificity and Catalytic Activity of an Enzyme; 9. Biological Fitness Landscapes by Deep Mutational Scanning; 10. Focused Rational Iterative Site-specific Mutagenesis (FRISM); 11. Statistical Analysis of Mutational Epistasis to Reveal Intramolecular Interaction Networks in Proteins; 12. Machine Learning-assisted Enzyme Engineering; 13. Ultrahigh Throughput Screening for Enzyme Function in Droplets.…